Emerald: Difference between revisions

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A GFP derivative, with 5X brighter than EGFP at 37C.  Largely due to improved folding.
A GFP derivative, with 5X brighter than EGFP at 37C.  Largely due to improved folding.
Excitation: 487 
Emission: 509


==Mutations==
==Mutations==
Line 8: Line 5:


==Spectra==
==Spectra==
*Excitation: 487 
*Emission: 509
[http://www.invitrogen.com/imgLibrary/vivid_graph1.jpg Excitation and Emission Spectra]
[http://www.invitrogen.com/imgLibrary/vivid_graph1.jpg Excitation and Emission Spectra]



Revision as of 09:12, 30 May 2006

A GFP derivative, with 5X brighter than EGFP at 37C. Largely due to improved folding.

Mutations

S65T, S72A, N149K, M153T, I167T [1]

Spectra

  • Excitation: 487
  • Emission: 509

Excitation and Emission Spectra

References

  1. Cubitt AB, Woollenweber LA, and Heim R. Understanding structure-function relationships in the Aequorea victoria green fluorescent protein. Methods Cell Biol. 1999;58:19-30. DOI:10.1016/s0091-679x(08)61946-9 | PubMed ID:9891372 | HubMed [cubitt]