Elizabeth Polidan Week11

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#trehalose
#trehalose
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#*A nonreducing disaccharide that acts as a storage carbohydrate and protects cells against various environmental stress conditions. 
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#*''Charlemagne-Gilles, H., et al., Role of trehalose in survival of Saccharomyces cerevisiae under osmotic stress, Microbiology (1998), 144, pp. 671-680. (http://mic.sgmjournals.org/content/144/3/671.full.pdf)''
#mannoproteins
#mannoproteins
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#*Yeast cell wall components composed of proteins with mannose groups attached.
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#*''http://www.biology-online.org/dictionary/Mannoproteins''
#prototrophic
#prototrophic
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#*A strain that has the same nutritional requirements as the wild strain from which it is derived. 
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#*''http://www.biology-online.org/dictionary/Prototrophic_strains''
#fatty-acid desaturase
#fatty-acid desaturase
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#*Enzymes that put double bonds into the hydrocarbon areas of fatty acids.
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#*''http://www.biology-online.org/dictionary/Desaturase''
#cis-regulatory motifs
#cis-regulatory motifs
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#*A region of DNA or RNA that regulates the expression of genes also located on that same strand of DNA or RNA. 
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#*''http://en.wikipedia.org/wiki/Cis-regulatory_element''
#HXT5
#HXT5
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#*Hexose transporter with moderate affinity for glucose.
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#*''http://www.yeastgenome.org/cgi-bin/locus.fpl?locus=HXT5''
#HXT6
#HXT6
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#*High-affinity glucose transporter 
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#*''http://www.yeastgenome.org/cgi-bin/locus.fpl?locus=hxt6''
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#Carbon recovery
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#*Ratio of amount of recovered carbon to the amount available at the onset of fermentation.  It is a measure of efficiency.  Usually percentage reflecting # moles produced per 100 moles of substrate used. 
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#*''El-Mansi, E.M.T, Bryce, C.F.A., Demain, A.L., and Allman, A.R. editors (2006) Fermentation Microbiology and Biothechnology, 2nd edition, Boca Raton, FL: CRC Press, p. 31.''
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'''Outline of Tai et al. (2007)'''
'''Outline of Tai et al. (2007)'''

Revision as of 22:26, 3 April 2013

alt=My children

Elizabeth Polidan

BIOL 398.03 / MATH 388

  • Loyola Marymount University
  • Los Angeles, CA, USA

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Vocabulary

  1. trehalose
    • A nonreducing disaccharide that acts as a storage carbohydrate and protects cells against various environmental stress conditions.
    • Charlemagne-Gilles, H., et al., Role of trehalose in survival of Saccharomyces cerevisiae under osmotic stress, Microbiology (1998), 144, pp. 671-680. (http://mic.sgmjournals.org/content/144/3/671.full.pdf)
  2. mannoproteins
  3. prototrophic
  4. fatty-acid desaturase
  5. cis-regulatory motifs
  6. HXT5
  7. HXT6
  8. Carbon recovery
    • Ratio of amount of recovered carbon to the amount available at the onset of fermentation.  It is a measure of efficiency.  Usually percentage reflecting # moles produced per 100 moles of substrate used. 
    • El-Mansi, E.M.T, Bryce, C.F.A., Demain, A.L., and Allman, A.R. editors (2006) Fermentation Microbiology and Biothechnology, 2nd edition, Boca Raton, FL: CRC Press, p. 31.


Outline of Tai et al. (2007)

  1. Introduction
    1. What is the main result presented in this paper?
    2. What is the importance or significance of this work?
  2. Methods
    1. How did they treat the cells (what experiment were they doing?)
    2. What strain(s) of yeast did they use? Was the strain haploid or diploid?
    3. What media did they grow them in? Under what conditions and temperatures?
    4. What controls did they use?
    5. How many replicates did they perform per condition?
  3. Analysis
    1. What mathematical/statistical method did they use to analyze the data?
  4. Discussion
    1. What transcription factors did they talk about?
    2. Briefly state the result shown in each of the figures and tables.

Placeholder for reflection questions.

Critically evaluate the Tai et al. (2007) paper.

  1. Overall, do you think this paper was clearly written? Why or why not?
  2. Based on what is written in the methods section, do you think you could reproduce their experiments and data analysis?
  3. What else would you like to know about their methods, results, and future directions?
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