Electroporation

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(Notes)
Current revision (13:40, 23 June 2009) (view source)
(Specific Protocols)
 
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*[[Knight:Electroporation]]
*[[Knight:Electroporation]]
*[[Endy:High-efficiency electroporation]]
*[[Endy:High-efficiency electroporation]]
 +
*[[Richard Lab:Electroporation of E. coli]]
==Notes==
==Notes==

Current revision

back to protocols

Specific Protocols

Notes

  • If you are in a hurry and your selection marker is ampicillin and you're sure to have enough plasmid (e.g. not after difficult clonings), you can go ahead and plate immediately because ampicillin takes a while to be pumped into cells at a high enough concentration to have an effect. This is called 5-minute transformation by New England Biolabs and if you follow the link you'll get a nice complete protocol for it (10% efficiency compared to full protocol)
  • The Endy Lab is trying to use a standard positive control to better compare (and hopefully improve) the transformation efficiencies in the lab, you can check it out here.
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