Electroporation
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==Specific Protocols== | ==Specific Protocols== | ||
*[[Knight:Electroporation]] | *[[Knight:Electroporation]] | ||
| - | + | *[[Endy:High-efficiency electroporation]] | |
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==Notes== | ==Notes== | ||
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*If you are in a hurry and your selection marker is ampicillin, you can go ahead and plate immediately because ampicillin takes a while to be pumped into cells at a high enough concentration to have an effect. | *If you are in a hurry and your selection marker is ampicillin, you can go ahead and plate immediately because ampicillin takes a while to be pumped into cells at a high enough concentration to have an effect. | ||
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*The [[Endy Lab]] is trying to use a standard positive control to better compare (and hopefully improve) the transformation efficiencies in the lab, you can check it out [[Endy:Standard transformation positive control|here]]. | *The [[Endy Lab]] is trying to use a standard positive control to better compare (and hopefully improve) the transformation efficiencies in the lab, you can check it out [[Endy:Standard transformation positive control|here]]. | ||
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[[Category:Protocol]] | [[Category:Protocol]] | ||
| + | [[Category:In vivo]] | ||
[[Category:Escherichia coli]] | [[Category:Escherichia coli]] | ||
Revision as of 17:17, 8 May 2007
Specific Protocols
Notes
- If you are in a hurry and your selection marker is ampicillin, you can go ahead and plate immediately because ampicillin takes a while to be pumped into cells at a high enough concentration to have an effect.
- The Endy Lab is trying to use a standard positive control to better compare (and hopefully improve) the transformation efficiencies in the lab, you can check it out here.
