Electro-transformation of Lactobacillus spp. - Revision history

Michael A. Speer: /* Contact */

Michael A. Speer — Fri, 16 Sep 2011 21:31:39 GMT

Contact

←Older revision		Revision as of 21:31, 16 September 2011
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	*Tom Richard (tlr20@psu.edu)		*Tom Richard (tlr20@psu.edu)
	*Mike Speer (mas853@psu.edu)		*Mike Speer (mas853@psu.edu)
-	*Matthew Orton (morto077@uottawa.ca)

	or instead, [[Talk:{{PAGENAME}}\|discuss this protocol]].		or instead, [[Talk:{{PAGENAME}}\|discuss this protocol]].

Michael A. Speer: /* Overview */

Michael A. Speer — Fri, 16 Sep 2011 21:29:26 GMT

Overview

←Older revision		Revision as of 21:29, 16 September 2011
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	While there is a wide variety of methods used for the electrotransformation of Lactobacillus spp., they all share a typical format.		While there is a wide variety of methods used for the electrotransformation of Lactobacillus spp., they all share a typical format.
-	*Dilution: This determines the amount of overnight culture to add to new ~~gorwth~~ media	+	*Dilution: This determines the amount of overnight culture to add to new growth media
	*Growth: These are the conditions used to grow the cells on the day you plan to transform.		*Growth: These are the conditions used to grow the cells on the day you plan to transform.
	*Washing: Cells are repeatedly centrifuged and resuspended in a solution of some type.		*Washing: Cells are repeatedly centrifuged and resuspended in a solution of some type.
	*Buffer: This is what is used for the final resuspension and electroporation.		*Buffer: This is what is used for the final resuspension and electroporation.
-	*Concentration: This determines how much buffer to add relative to the volume of the growth media.	+	*Concentration: This determines how much Electroporation buffer to add relative to the volume of the growth media.
	*Voltage: How hard to shock em.		*Voltage: How hard to shock em.
	*Recovery: What to do with your cells after the shock.		*Recovery: What to do with your cells after the shock.

Michael A. Speer: /* References */

Michael A. Speer — Fri, 16 Sep 2011 21:28:00 GMT

References

←Older revision		Revision as of 21:28, 16 September 2011
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	*Serror, P., Sasaki, T., Ehrlich, S.D. and Emmanuelle Maguin. (2002) "Electrotransformation of ''Lactobacillus delbrueckii'' subsp. bulgaricus and ''L. delbrueckii'' subsp. lactis with Various Plasmids." ''Applied and Environmental Microbiology'', p.46–52		*Serror, P., Sasaki, T., Ehrlich, S.D. and Emmanuelle Maguin. (2002) "Electrotransformation of ''Lactobacillus delbrueckii'' subsp. bulgaricus and ''L. delbrueckii'' subsp. lactis with Various Plasmids." ''Applied and Environmental Microbiology'', p.46–52
		+
		+	*Speer, M.A. and T.L. Richard (2012) Will Soon Exist.

	*Thompson, K. and M. A. Collins (1996). "Improvement in electroporation efficiency for ''Lactobacillus plantarum'' by the inclusion of high concentrations of glycine in the growth medium." ''Journal of Microbiological Methods'' 26(1-2): 73-79.		*Thompson, K. and M. A. Collins (1996). "Improvement in electroporation efficiency for ''Lactobacillus plantarum'' by the inclusion of high concentrations of glycine in the growth medium." ''Journal of Microbiological Methods'' 26(1-2): 73-79.

Michael A. Speer: /* References */

Michael A. Speer — Fri, 16 Sep 2011 21:26:11 GMT

References

←Older revision		Revision as of 21:26, 16 September 2011
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	*Josson, K., T. Scheirlinck, et al. (1989). "Characterization of a gram-positive broad-host-range plasmid isolated from Lactobacillus hilgardii." ''Plasmid'' 21(1): 9-20.		*Josson, K., T. Scheirlinck, et al. (1989). "Characterization of a gram-positive broad-host-range plasmid isolated from Lactobacillus hilgardii." ''Plasmid'' 21(1): 9-20.

-	*Kim, Y.H., Han, K.S., Oh, s., You, S. and S.H. Kim (2005) "Optimization of technical conditions for the transformation of	+	*Kim, Y.H., Han, K.S., Oh, s., You, S. and S.H. Kim (2005) "Optimization of technical conditions for the transformation of Lactobacillus acidophilus strains by electroporation." ''Journal of Applied Microbiology'' 99: 167–174
-	Lactobacillus acidophilus strains by electroporation." ''Journal of Applied Microbiology'' 99: 167–174	+

	*Mason, C. K., M. A. Collins, et al. (2005). "Modified electroporation protocol for Lactobacilli isolated frorn the chicken crop facilitates transformation and the use of a genetic tool." ''Journal of Microbiological Methods'' 60(3): 353-363.		*Mason, C. K., M. A. Collins, et al. (2005). "Modified electroporation protocol for Lactobacilli isolated frorn the chicken crop facilitates transformation and the use of a genetic tool." ''Journal of Microbiological Methods'' 60(3): 353-363.

Michael A. Speer: /* References */

Michael A. Speer — Fri, 16 Sep 2011 21:25:51 GMT

References

←Older revision		Revision as of 21:25, 16 September 2011
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	*Josson, K., T. Scheirlinck, et al. (1989). "Characterization of a gram-positive broad-host-range plasmid isolated from Lactobacillus hilgardii." ''Plasmid'' 21(1): 9-20.		*Josson, K., T. Scheirlinck, et al. (1989). "Characterization of a gram-positive broad-host-range plasmid isolated from Lactobacillus hilgardii." ''Plasmid'' 21(1): 9-20.

-	Kim, Y.H., Han, K.S., Oh, s., You, S. and S.H. Kim (2005) "Optimization of technical conditions for the transformation of	+	*Kim, Y.H., Han, K.S., Oh, s., You, S. and S.H. Kim (2005) "Optimization of technical conditions for the transformation of
	Lactobacillus acidophilus strains by electroporation." ''Journal of Applied Microbiology'' 99: 167–174		Lactobacillus acidophilus strains by electroporation." ''Journal of Applied Microbiology'' 99: 167–174

Michael A. Speer: /* References */

Michael A. Speer — Fri, 16 Sep 2011 21:25:39 GMT

References

←Older revision		Revision as of 21:25, 16 September 2011
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	*Josson, K., T. Scheirlinck, et al. (1989). "Characterization of a gram-positive broad-host-range plasmid isolated from Lactobacillus hilgardii." ''Plasmid'' 21(1): 9-20.		*Josson, K., T. Scheirlinck, et al. (1989). "Characterization of a gram-positive broad-host-range plasmid isolated from Lactobacillus hilgardii." ''Plasmid'' 21(1): 9-20.
		+
		+	Kim, Y.H., Han, K.S., Oh, s., You, S. and S.H. Kim (2005) "Optimization of technical conditions for the transformation of
		+	Lactobacillus acidophilus strains by electroporation." ''Journal of Applied Microbiology'' 99: 167–174

	*Mason, C. K., M. A. Collins, et al. (2005). "Modified electroporation protocol for Lactobacilli isolated frorn the chicken crop facilitates transformation and the use of a genetic tool." ''Journal of Microbiological Methods'' 60(3): 353-363.		*Mason, C. K., M. A. Collins, et al. (2005). "Modified electroporation protocol for Lactobacilli isolated frorn the chicken crop facilitates transformation and the use of a genetic tool." ''Journal of Microbiological Methods'' 60(3): 353-363.

Michael A. Speer: /* Contact */

Michael A. Speer — Fri, 16 Sep 2011 21:22:19 GMT

Contact

←Older revision		Revision as of 21:22, 16 September 2011
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	==Contact==		==Contact==
-	*tlr20@psu.edu	+	*Tom Richard (tlr20@psu.edu)
-	*mas853@psu.edu	+	*Mike Speer (mas853@psu.edu)
-	*morto077@uottawa.ca	+	*Matthew Orton (morto077@uottawa.ca)

	or instead, [[Talk:{{PAGENAME}}\|discuss this protocol]].		or instead, [[Talk:{{PAGENAME}}\|discuss this protocol]].

Michael A. Speer: /* Contact */

Michael A. Speer — Fri, 16 Sep 2011 21:20:23 GMT

Contact

←Older revision		Revision as of 21:20, 16 September 2011
Line 164:		Line 164:

	==Contact==		==Contact==
-	*~~morto077~~@~~uottawa~~.ca	+	*tlr20@psu.edu
-		+
	*mas853@psu.edu		*mas853@psu.edu
-		+	*morto077@uottawa.ca

	or instead, [[Talk:{{PAGENAME}}\|discuss this protocol]].		or instead, [[Talk:{{PAGENAME}}\|discuss this protocol]].

Michael A. Speer: /* References */

Michael A. Speer — Fri, 16 Sep 2011 21:19:40 GMT

References

←Older revision		Revision as of 21:19, 16 September 2011
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	*Alegre, M.T, Rodrıguez, M.C., and J.M. Mesas. (2004) "Transformation of Lactobacillus plantarum by electroporation with in vitro modified plasmid DNA." ''FEMS Microbiology Letters'' 241: 73-77)		*Alegre, M.T, Rodrıguez, M.C., and J.M. Mesas. (2004) "Transformation of Lactobacillus plantarum by electroporation with in vitro modified plasmid DNA." ''FEMS Microbiology Letters'' 241: 73-77)

-	*Aukrust, T. and H. Blom (1992). "Transformation of Lactobacillus strains used in meat and vegetable fermentaions." Food Research International 25(4): 253-261.	+	*Aukrust, T. and H. Blom (1992). "Transformation of Lactobacillus strains used in meat and vegetable fermentaions." ''Food Research International'' 25(4): 253-261.

-	*Berthier, F., M. Zagorec, et al. (1996). "Efficient transformation of ''Lactobacillus sake'' by electroporation." Microbiology-Uk 142: 1273-1279.	+	*Berthier, F., M. Zagorec, et al. (1996). "Efficient transformation of ''Lactobacillus sake'' by electroporation." ''Microbiology-Uk'' 142: 1273-1279.

-	*Bringel, F. and J.C. Hubert. (1990) "Optimized transformation by electroporation of ''Lactobacillus plantarum'' strains with plasmid vectors" Applied Microbiology and Biotechnology 33:664-670	+	*Bringel, F. and J.C. Hubert. (1990) "Optimized transformation by electroporation of ''Lactobacillus plantarum'' strains with plasmid vectors" ''Applied Microbiology and Biotechnology'' 33:664-670

-	*Josson, K., T. Scheirlinck, et al. (1989). "Characterization of a gram-positive broad-host-range plasmid isolated from Lactobacillus hilgardii." Plasmid 21(1): 9-20.	+	*Josson, K., T. Scheirlinck, et al. (1989). "Characterization of a gram-positive broad-host-range plasmid isolated from Lactobacillus hilgardii." ''Plasmid'' 21(1): 9-20.

-	*Mason, C. K., M. A. Collins, et al. (2005). "Modified electroporation protocol for Lactobacilli isolated frorn the chicken crop facilitates transformation and the use of a genetic tool." Journal of Microbiological Methods 60(3): 353-363.	+	*Mason, C. K., M. A. Collins, et al. (2005). "Modified electroporation protocol for Lactobacilli isolated frorn the chicken crop facilitates transformation and the use of a genetic tool." ''Journal of Microbiological Methods'' 60(3): 353-363.

-	*Posno, M., R. J. Leer, et al. (1991). "Incompatibility of ''Lactobacillus'' vectors with replicons derived from small cryptic plasmids and segregational instability of the introduced vectors." Applied and Environmental Microbiology 57(6): 1822-1828.	+	*Posno, M., R. J. Leer, et al. (1991). "Incompatibility of ''Lactobacillus'' vectors with replicons derived from small cryptic plasmids and segregational instability of the introduced vectors." ''Applied and Environmental Microbiology'' 57(6): 1822-1828.

	*Serror, P., Sasaki, T., Ehrlich, S.D. and Emmanuelle Maguin. (2002) "Electrotransformation of ''Lactobacillus delbrueckii'' subsp. bulgaricus and ''L. delbrueckii'' subsp. lactis with Various Plasmids." ''Applied and Environmental Microbiology'', p.46–52		*Serror, P., Sasaki, T., Ehrlich, S.D. and Emmanuelle Maguin. (2002) "Electrotransformation of ''Lactobacillus delbrueckii'' subsp. bulgaricus and ''L. delbrueckii'' subsp. lactis with Various Plasmids." ''Applied and Environmental Microbiology'', p.46–52

-	*Thompson, K. and M. A. Collins (1996). "Improvement in electroporation efficiency for ''Lactobacillus plantarum'' by the inclusion of high concentrations of glycine in the growth medium." Journal of Microbiological Methods 26(1-2): 73-79.	+	*Thompson, K. and M. A. Collins (1996). "Improvement in electroporation efficiency for ''Lactobacillus plantarum'' by the inclusion of high concentrations of glycine in the growth medium." ''Journal of Microbiological Methods'' 26(1-2): 73-79.

-	*Wei, M. Q., C. M. Rush, et al. (1995). "An improved method for the transformation of ''Lactobacillus'' strains using electroporation." Journal of Microbiological Methods 21(1): 97-109.	+	*Wei, M. Q., C. M. Rush, et al. (1995). "An improved method for the transformation of ''Lactobacillus'' strains using electroporation." ''Journal of Microbiological Methods'' 21(1): 97-109.

	==Contact==		==Contact==

Michael A. Speer: /* References */

Michael A. Speer — Fri, 16 Sep 2011 21:18:28 GMT

References

←Older revision		Revision as of 21:18, 16 September 2011
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	==References==		==References==

-	*Alegre, M.T, Rodrıguez, M.C., and J.M. Mesas. (2004) "Transformation of Lactobacillus plantarum by electroporation	+	*Alegre, M.T, Rodrıguez, M.C., and J.M. Mesas. (2004) "Transformation of Lactobacillus plantarum by electroporation with in vitro modified plasmid DNA." ''FEMS Microbiology Letters'' 241: 73-77)
-	with in vitro modified plasmid DNA." ''FEMS Microbiology Letters'' 241: 73-77)	+

	*Aukrust, T. and H. Blom (1992). "Transformation of Lactobacillus strains used in meat and vegetable fermentaions." Food Research International 25(4): 253-261.		*Aukrust, T. and H. Blom (1992). "Transformation of Lactobacillus strains used in meat and vegetable fermentaions." Food Research International 25(4): 253-261.