EcoRI - Revision history

Karmella Haynes: /* Notes */

Karmella Haynes — Thu, 19 Jan 2012 19:07:31 GMT

Notes

←Older revision		Revision as of 19:07, 19 January 2012
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	* '''Reaction Volumes''' - I used to have very inconsistent results when digesting with EcoRI, using the 20μl reaction volume. Using the Knight Lab's 50μl mix has recently given good results. I haven't tested this enough to say it is a statistically significant result. --[[User:Bcanton\|BC]] 13:39, 2 Jun 2005 (EDT)		* '''Reaction Volumes''' - I used to have very inconsistent results when digesting with EcoRI, using the 20μl reaction volume. Using the Knight Lab's 50μl mix has recently given good results. I haven't tested this enough to say it is a statistically significant result. --[[User:Bcanton\|BC]] 13:39, 2 Jun 2005 (EDT)
	* The custom EcoRI buffer provided by NEB contains Triton-X100. This detergent is very inhibitory in transformations, so direct ligation and cloning of DNA cut in this buffer is problematic. Remarkably small amounts of this buffer dramatically reduces transformation efficiency.		* The custom EcoRI buffer provided by NEB contains Triton-X100. This detergent is very inhibitory in transformations, so direct ligation and cloning of DNA cut in this buffer is problematic. Remarkably small amounts of this buffer dramatically reduces transformation efficiency.
		+	* The FastDigest restriction enzymes from Fermentas all work very efficiently in one universal buffer. I perform digests in a 30 μL reaction volume. *'''[[User:Karmella Haynes\|Karmella Haynes]] 14:07, 19 January 2012 (EST)''':

	==External links==		==External links==

Reshma P. Shetty: /* References */

Reshma P. Shetty — Thu, 20 Dec 2007 20:52:07 GMT

References

←Older revision		Revision as of 20:52, 20 December 2007
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	#Mertz-Proc-Natl-Acad-Sci-USA-1972 pmid=4343968		#Mertz-Proc-Natl-Acad-Sci-USA-1972 pmid=4343968
	// Use of EcoRI to generate DNA fragments that can be ligated		// Use of EcoRI to generate DNA fragments that can be ligated
-	#Cohen-PNAS-~~1972~~ pmid=4594039	+	#Cohen-PNAS-1973 pmid=4594039
	// Use of EcoRI to generate recombinant DNA fragments		// Use of EcoRI to generate recombinant DNA fragments
	</biblio>		</biblio>

	[[Category:Material]] [[Category:Enzyme]] [[Category:Endonuclease]]		[[Category:Material]] [[Category:Enzyme]] [[Category:Endonuclease]]

Reshma P. Shetty: /* References */

Reshma P. Shetty — Thu, 20 Dec 2007 20:51:53 GMT

References

←Older revision		Revision as of 20:51, 20 December 2007
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	// No abstract available online.		// No abstract available online.
	#Mertz-Proc-Natl-Acad-Sci-USA-1972 pmid=4343968		#Mertz-Proc-Natl-Acad-Sci-USA-1972 pmid=4343968
		+	// Use of EcoRI to generate DNA fragments that can be ligated
		+	#Cohen-PNAS-1972 pmid=4594039
	// Use of EcoRI to generate recombinant DNA fragments		// Use of EcoRI to generate recombinant DNA fragments
	</biblio>		</biblio>

	[[Category:Material]] [[Category:Enzyme]] [[Category:Endonuclease]]		[[Category:Material]] [[Category:Enzyme]] [[Category:Endonuclease]]

Reshma P. Shetty: /* References */

Reshma P. Shetty — Thu, 20 Dec 2007 20:48:20 GMT

References

←Older revision		Revision as of 20:48, 20 December 2007
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	==References==		==References==
	<biblio>		<biblio>
		+	#Morrow-PNAS-1972 pmid=4343967
		+	// Discusses cleavage of DNA at a unique location by EcoRI
		+	#Mulder-PNAS-1972 pmid=4343959
		+	// Specificity of EcoRI
	#Hedgpeth-Proc-Natl-Acad-Sci-USA-1972 pmid=4343974		#Hedgpeth-Proc-Natl-Acad-Sci-USA-1972 pmid=4343974
	// Identifies the overhang sequence produced by EcoRI		// Identifies the overhang sequence produced by EcoRI

Reshma P. Shetty at 20:43, 20 December 2007

Reshma P. Shetty — Thu, 20 Dec 2007 20:43:22 GMT

←Older revision		Revision as of 20:43, 20 December 2007
Line 11:		Line 11:
	* The custom EcoRI buffer provided by NEB contains Triton-X100. This detergent is very inhibitory in transformations, so direct ligation and cloning of DNA cut in this buffer is problematic. Remarkably small amounts of this buffer dramatically reduces transformation efficiency.		* The custom EcoRI buffer provided by NEB contains Triton-X100. This detergent is very inhibitory in transformations, so direct ligation and cloning of DNA cut in this buffer is problematic. Remarkably small amounts of this buffer dramatically reduces transformation efficiency.

-	==~~References~~==	+	==External links==
	[http://www.neb.com/nebecomm/products/productR0101.asp EcoRI from NEB]<br />		[http://www.neb.com/nebecomm/products/productR0101.asp EcoRI from NEB]<br />
	[http://www.promega.com/catalog/catalogproducts.asp?catalog_name=Promega_Products&category_name=EcoR+I&cookie%5Ftest=1 EcoRI from Promega]<br />		[http://www.promega.com/catalog/catalogproducts.asp?catalog_name=Promega_Products&category_name=EcoR+I&cookie%5Ftest=1 EcoRI from Promega]<br />
		+
		+	==References==
		+	<biblio>
		+	#Hedgpeth-Proc-Natl-Acad-Sci-USA-1972 pmid=4343974
		+	// Identifies the overhang sequence produced by EcoRI
		+	#Bigger-Nat-New-Biol-1973 pmid=4578426
		+	// No abstract available online.
		+	#Mertz-Proc-Natl-Acad-Sci-USA-1972 pmid=4343968
		+	// Use of EcoRI to generate recombinant DNA fragments
		+	</biblio>

	[[Category:Material]] [[Category:Enzyme]] [[Category:Endonuclease]]		[[Category:Material]] [[Category:Enzyme]] [[Category:Endonuclease]]

Reshma P. Shetty at 17:06, 27 May 2007

Reshma P. Shetty — Sun, 27 May 2007 17:06:20 GMT

←Older revision		Revision as of 17:06, 27 May 2007
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	[http://www.neb.com/nebecomm/products/productR0101.asp EcoRI from NEB]<br />		[http://www.neb.com/nebecomm/products/productR0101.asp EcoRI from NEB]<br />
	[http://www.promega.com/catalog/catalogproducts.asp?catalog_name=Promega_Products&category_name=EcoR+I&cookie%5Ftest=1 EcoRI from Promega]<br />		[http://www.promega.com/catalog/catalogproducts.asp?catalog_name=Promega_Products&category_name=EcoR+I&cookie%5Ftest=1 EcoRI from Promega]<br />
		+
		+	[[Category:Material]] [[Category:Enzyme]] [[Category:Endonuclease]]

Tk: /* Notes */

Tk — Tue, 12 Sep 2006 21:41:01 GMT

Notes

←Older revision		Revision as of 21:41, 12 September 2006
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	==Notes==		==Notes==
-	*Improving the efficiency of [[EcoRI/SpeI Double Digest]].	+	* Improving the efficiency of [[EcoRI/SpeI Double Digest]].
-	*'''Reaction Volumes''' - I used to have very inconsistent results when digesting with EcoRI, using the 20μl reaction volume. Using the Knight Lab's 50μl mix has recently given good results. I haven't tested this enough to say it is a statistically significant result. --[[User:Bcanton\|BC]] 13:39, 2 Jun 2005 (EDT)	+	* '''Reaction Volumes''' - I used to have very inconsistent results when digesting with EcoRI, using the 20μl reaction volume. Using the Knight Lab's 50μl mix has recently given good results. I haven't tested this enough to say it is a statistically significant result. --[[User:Bcanton\|BC]] 13:39, 2 Jun 2005 (EDT)
		+	* The custom EcoRI buffer provided by NEB contains Triton-X100. This detergent is very inhibitory in transformations, so direct ligation and cloning of DNA cut in this buffer is problematic. Remarkably small amounts of this buffer dramatically reduces transformation efficiency.

	==References==		==References==
	[http://www.neb.com/nebecomm/products/productR0101.asp EcoRI from NEB]<br />		[http://www.neb.com/nebecomm/products/productR0101.asp EcoRI from NEB]<br />
	[http://www.promega.com/catalog/catalogproducts.asp?catalog_name=Promega_Products&category_name=EcoR+I&cookie%5Ftest=1 EcoRI from Promega]<br />		[http://www.promega.com/catalog/catalogproducts.asp?catalog_name=Promega_Products&category_name=EcoR+I&cookie%5Ftest=1 EcoRI from Promega]<br />

Trunkflare: /* References */

Trunkflare — Fri, 02 Jun 2006 18:12:56 GMT

References

←Older revision		Revision as of 18:12, 2 June 2006
Line 11:		Line 11:

	==References==		==References==
-	[http://www.neb.com/nebecomm/products/productR0101.asp EcoRI from NEB]	+	[http://www.neb.com/nebecomm/products/productR0101.asp EcoRI from NEB]<br />
-	[http://www.promega.com/catalog/catalogproducts.asp?catalog_name=Promega_Products&category_name=EcoR+I&cookie%5Ftest=1 EcoRI from Promega]	+	[http://www.promega.com/catalog/catalogproducts.asp?catalog_name=Promega_Products&category_name=EcoR+I&cookie%5Ftest=1 EcoRI from Promega]<br />

Trunkflare: /* References */

Trunkflare — Fri, 02 Jun 2006 18:12:32 GMT

References

←Older revision		Revision as of 18:12, 2 June 2006
Line 12:		Line 12:
	==References==		==References==
	[http://www.neb.com/nebecomm/products/productR0101.asp EcoRI from NEB]		[http://www.neb.com/nebecomm/products/productR0101.asp EcoRI from NEB]
		+	[http://www.promega.com/catalog/catalogproducts.asp?catalog_name=Promega_Products&category_name=EcoR+I&cookie%5Ftest=1 EcoRI from Promega]

Reshma P. Shetty at 14:57, 3 May 2006

Reshma P. Shetty — Wed, 03 May 2006 14:57:12 GMT

←Older revision		Revision as of 14:57, 3 May 2006
Line 1:		Line 1:
		+	==Properties==
		+	===Recognition site===
		+	http://www.neb.com/nebecomm/productfiles/314/images/EcoR-I-cutsite_1.gif
		+
		+	===Buffers===
		+	NEBuffer EcoR I
		+
		+	==Notes==
	*Improving the efficiency of [[EcoRI/SpeI Double Digest]].		*Improving the efficiency of [[EcoRI/SpeI Double Digest]].
	*'''Reaction Volumes''' - I used to have very inconsistent results when digesting with EcoRI, using the 20μl reaction volume. Using the Knight Lab's 50μl mix has recently given good results. I haven't tested this enough to say it is a statistically significant result. --[[User:Bcanton\|BC]] 13:39, 2 Jun 2005 (EDT)		*'''Reaction Volumes''' - I used to have very inconsistent results when digesting with EcoRI, using the 20μl reaction volume. Using the Knight Lab's 50μl mix has recently given good results. I haven't tested this enough to say it is a statistically significant result. --[[User:Bcanton\|BC]] 13:39, 2 Jun 2005 (EDT)
		+
		+	==References==
		+	[http://www.neb.com/nebecomm/products/productR0101.asp EcoRI from NEB]