EcoRI

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==References==
==References==
[http://www.neb.com/nebecomm/products/productR0101.asp EcoRI from NEB]
[http://www.neb.com/nebecomm/products/productR0101.asp EcoRI from NEB]
 +
[http://www.promega.com/catalog/catalogproducts.asp?catalog_name=Promega_Products&category_name=EcoR+I&cookie%5Ftest=1 EcoRI from Promega]

Revision as of 14:12, 2 June 2006

Contents

Properties

Recognition site

EcoR-I-cutsite_1.gif

Buffers

NEBuffer EcoR I

Notes

  • Improving the efficiency of EcoRI/SpeI Double Digest.
  • Reaction Volumes - I used to have very inconsistent results when digesting with EcoRI, using the 20μl reaction volume. Using the Knight Lab's 50μl mix has recently given good results. I haven't tested this enough to say it is a statistically significant result. --BC 13:39, 2 Jun 2005 (EDT)

References

EcoRI from NEB EcoRI from Promega

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