EcoRI

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==Properties==
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===Recognition site===
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http://www.neb.com/nebecomm/productfiles/314/images/EcoR-I-cutsite_1.gif
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===Buffers===
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NEBuffer EcoR I
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==Notes==
*Improving the efficiency of [[EcoRI/SpeI Double Digest]].
*Improving the efficiency of [[EcoRI/SpeI Double Digest]].
*'''Reaction Volumes''' - I used to have very inconsistent results when digesting with EcoRI, using the 20μl reaction volume. Using the Knight Lab's 50μl mix has recently given good results.  I haven't tested this enough to say it is a statistically significant result.  --[[User:Bcanton|BC]] 13:39, 2 Jun 2005 (EDT)
*'''Reaction Volumes''' - I used to have very inconsistent results when digesting with EcoRI, using the 20μl reaction volume. Using the Knight Lab's 50μl mix has recently given good results.  I haven't tested this enough to say it is a statistically significant result.  --[[User:Bcanton|BC]] 13:39, 2 Jun 2005 (EDT)
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==References==
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[http://www.neb.com/nebecomm/products/productR0101.asp EcoRI from NEB]

Revision as of 09:57, 3 May 2006

Contents

Properties

Recognition site

EcoR-I-cutsite_1.gif

Buffers

NEBuffer EcoR I

Notes

  • Improving the efficiency of EcoRI/SpeI Double Digest.
  • Reaction Volumes - I used to have very inconsistent results when digesting with EcoRI, using the 20μl reaction volume. Using the Knight Lab's 50μl mix has recently given good results. I haven't tested this enough to say it is a statistically significant result. --BC 13:39, 2 Jun 2005 (EDT)

References

EcoRI from NEB

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