EcoRI
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==References== | ==References== | ||
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| + | #Morrow-PNAS-1972 pmid=4343967 | ||
| + | // Discusses cleavage of DNA at a unique location by EcoRI | ||
| + | #Mulder-PNAS-1972 pmid=4343959 | ||
| + | // Specificity of EcoRI | ||
#Hedgpeth-Proc-Natl-Acad-Sci-USA-1972 pmid=4343974 | #Hedgpeth-Proc-Natl-Acad-Sci-USA-1972 pmid=4343974 | ||
// Identifies the overhang sequence produced by EcoRI | // Identifies the overhang sequence produced by EcoRI | ||
Revision as of 16:48, 20 December 2007
Contents |
Properties
Recognition site
Buffers
NEBuffer EcoR I
Notes
- Improving the efficiency of EcoRI/SpeI Double Digest.
- Reaction Volumes - I used to have very inconsistent results when digesting with EcoRI, using the 20μl reaction volume. Using the Knight Lab's 50μl mix has recently given good results. I haven't tested this enough to say it is a statistically significant result. --BC 13:39, 2 Jun 2005 (EDT)
- The custom EcoRI buffer provided by NEB contains Triton-X100. This detergent is very inhibitory in transformations, so direct ligation and cloning of DNA cut in this buffer is problematic. Remarkably small amounts of this buffer dramatically reduces transformation efficiency.
External links
EcoRI from NEB
EcoRI from Promega
References
- Morrow JF and Berg P. . pmid:4343967.
Discusses cleavage of DNA at a unique location by EcoRI - Mulder C and Delius H. . pmid:4343959.
Specificity of EcoRI - Hedgpeth J, Goodman HM, and Boyer HW. . pmid:4343974.
Identifies the overhang sequence produced by EcoRI - Bigger CH, Murray K, and Murray NE. . pmid:4578426.
No abstract available online. - Mertz JE and Davis RW. . pmid:4343968.
Use of EcoRI to generate recombinant DNA fragments
