EGTA
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| - | '''EGTA''' | + | '''Ethylene glycol tetraacetic acid (EGTA)''' is a common buffer ingredient due to its chelating activity. It is similar to the better known [[EDTA]], but has a much higher affinity for calcium ions than for magnesium ions. Buffers made with EGTA are used in some cases to mimic the environment inside living cells where calcium ions are usually at least a thousandfold less concentrated than magnesium ions. |
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| + | Full chemical name: ethylene glycol-bis(2-aminoethylether)-N,N,N',N'-tetraacetic acid | ||
== EGTA 0.5M stock solution == | == EGTA 0.5M stock solution == | ||
Revision as of 09:28, 15 June 2009
Ethylene glycol tetraacetic acid (EGTA) is a common buffer ingredient due to its chelating activity. It is similar to the better known EDTA, but has a much higher affinity for calcium ions than for magnesium ions. Buffers made with EGTA are used in some cases to mimic the environment inside living cells where calcium ions are usually at least a thousandfold less concentrated than magnesium ions.
Full chemical name: ethylene glycol-bis(2-aminoethylether)-N,N,N',N'-tetraacetic acid
EGTA 0.5M stock solution
100 ml solution
- 23.4g EGTA
- ddH2O
- pH 7.5/8.0
