EDTA

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EDTA (ethylenediaminetetraacetic acid) is a synthetic amino acid.
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*Most enzymes that synthesize or modify nucleic acids (e.g. polymerases, ligases, kinases, nucleases) are Mg<sup>2+</sup>-dependent. The addition of EDTA is a convenient way to stop these reactions.
*Most enzymes that synthesize or modify nucleic acids (e.g. polymerases, ligases, kinases, nucleases) are Mg<sup>2+</sup>-dependent. The addition of EDTA is a convenient way to stop these reactions.

Revision as of 13:01, 15 April 2006

EDTA (ethylenediaminetetraacetic acid) is a synthetic amino acid.

  • Most enzymes that synthesize or modify nucleic acids (e.g. polymerases, ligases, kinases, nucleases) are Mg2+-dependent. The addition of EDTA is a convenient way to stop these reactions.
  • Here's a great page about EDTA, including formation constant (Kf) values for metal-EDTA complexes. Note that many heavy metal ions (like Fe3+, Co2+, and Zn2+) are chelated much more strongly than Mg2+. A little bit of EDTA in your reaction will go a long way to keep these evildoers out of trouble and away from your precious biomolecules without interfering with your Mg2+-dependent reactions.
  • Here's another article that speaks of the wonders of EDTA and how it can help treat Aunt Edna's cardiovascular disease, enhance your enjoyment of the Friday night happy hour, and act as a "get out of jail free" card.
  • EDTA helps you determine the hidden contents of your hard water.
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