E. huxleyi PCR: Difference between revisions

Latest revision as of 07:51, 11 September 2006

HOW TO ... PCR Rutgers, October 2004

1- Reagents needed (volume in each PCR tube)

All must be kept frozen and kept on ice during manipulations

- PCR buffer (2.5 µl)

- 10 nM dNTP's 10 nM (1.25 µl) [must not be thawed more than 25 times]

- MgSO4 (0.75 µl)

- 10 nM primer 1 (0.5 µl) [must not be thawed more than 25 times]

- 10 nM primer 2 (0.5 µl) [must not be thawed more than 25 times]

- RedTaq (1.25 µl)

- HPLC grade H2O (14.75 µl)

- 10x PCR enhancer (2.5 µl)

2-

@@ Line 3: / Line 3: @@
 - Reagents needed (volume in each PCR tube)
 All must be kept frozen and kept on ice during manipulations
-- PCR buffer				(2.5 µl)
-- 10 nM dNTP's 10 nM	(1.25 µl) [must not be thawed more than 25 times]
+- PCR buffer (2.5 µl)
-- MgSO4					(0.75 µl)
-- 10 nM primer 1		(0.5 µl) [must not be thawed more than 25 times]
+- 10 nM dNTP's 10 nM (1.25 µl) [must not be thawed more than 25 times]
-- 10 nM primer 2		(0.5 µl) [must not be thawed more than 25 times]
-- RedTaq						(1.25 µl)
+- MgSO4 (0.75 µl)
-- HPLC grade H2O 		(14.75 µl)
-- 10x PCR enhancer		(2.5 µl)
+- 10 nM primer 1 (0.5 µl) [must not be thawed more than 25 times]
+- 10 nM primer 2 (0.5 µl) [must not be thawed more than 25 times]
+- RedTaq (1.25 µl)
+- HPLC grade H2O (14.75 µl)
+- 10x PCR enhancer (2.5 µl)
 -