E. coli genotypes/Exhibit: Difference between revisions
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|prophage | |prophage | ||
|- | |- | ||
|BL21(DE3) pLysS | |BL21(DE3) pLysS | ||
|The pLysS plasmid (with chloramphenicol resistance) encodes T7 phage lysozyme, an inhibitor for T7 polymerase which reduces and almost eliminates expression from transformed T7 promoter containing plasmids when not induced. | |The pLysS plasmid (with chloramphenicol resistance) encodes T7 phage lysozyme, an inhibitor for T7 polymerase which reduces and almost eliminates expression from transformed T7 promoter containing plasmids when not induced. | ||
|F-;ompT;gal;dcm;lon;hsdSB(rB- mB-);lacIq;lambda;lacUV5-T7 gene 1;ind1;sam7;nin5;pLysS(cmR) | |F-;ompT;gal;dcm;lon;hsdSB(rB- mB-);lacIq;lambda;lacUV5-T7 gene 1;ind1;sam7;nin5;pLysS(cmR) | ||
Revision as of 14:40, 24 September 2007
This is a demo of Exhibit. It only includes some of the data from E. coli genotypes.
<exhibit>
<source id="ecoligenotypes"
columns="label,genotype,parent,requires,resistance,purpose,inducible,lambda"
type="Strain" label="Strain" pluralLabel="Strains"
/>
<facet facetClass="TextSearch" />
<facet expression=".purpose" />
<facet expression=".resistance" />
<facet expression=".requires" />
<facet expression=".genotype" />
<view viewClass="Tabular"
label="Table"
columns=".label, .genotype, .parent, .purpose"
sortColumn="0"
/>
</exhibit>
| Strain | Description | Genotype | Parent | Requires | Resistance | Purpose | Inducible | Lambda |
|---|---|---|---|---|---|---|---|---|
| AG1 | endA1;recA1;gyrA96;thi-1;relA1;glnV44;hsdR17(rK- mK+) | thiamine | nalidixic acid | cloning | ||||
| BL21(DE3) | F-;ompT;gal;dcm;lon;hsdSB(rB- mB-);lacIq;lambda;lacUV5-T7 gene 1;ind1;sam7;nin5 | B834 | protein expression | T7 RNA polymerase | prophage | |||
| BL21(DE3) pLysS | The pLysS plasmid (with chloramphenicol resistance) encodes T7 phage lysozyme, an inhibitor for T7 polymerase which reduces and almost eliminates expression from transformed T7 promoter containing plasmids when not induced. | F-;ompT;gal;dcm;lon;hsdSB(rB- mB-);lacIq;lambda;lacUV5-T7 gene 1;ind1;sam7;nin5;pLysS(cmR) | BL21(DE3) | chloramphenicol | protein expression | T7 RNA polymerase | prophage |
<html> <script type="text/javascript"> /* var data = {
[
{
label : "BNN93",
lambda : "no",
requires : ["thiamine", "threonine", "leucine"],
genotype : ["F-", "tonA21", "thi-1", "thr-1", "leuB6", "lacY1", "glnV44", "rfbC1", "fhuA1", "mcrB", "e14-(mcrA-)", "hsdR(rKmK)", "lambda-"]
},
{
label : "BW26434 CGSC Strain #7658",
lambda : "no",
resistance : "kanamycin",
genotype : ["D(araD-araB)567", "D(lacA-lacZ)514(::kan)", "lacIp-4000(lacIq)", "lambda-", "rpoS396(Am)?", "rph-1", "D(rhaD-rhaB)568", "hsdR514"]
},
{
label : "C600",
purpose : "general purpose",
lambda : "no",
requires : ["thiamine", "threonine", "leucine"],
genotype : ["F-", "tonA21", "thi-1", "thr-1", "leuB6", "lacY1", "glnV44", "rfbC1", "fhuA1", "lambda-"]
},
{
label : "C600 hflA150 (Y1073, BNN102)",
parent : "C600",
purpose : "cDNA libraries",
lambda : "no",
resistance : "tetracycline",
requires : ["thiamine", "threonine", "leucine"],
genotype : ["F-", "thi-1", "thr-1", "leuB6", "lacY1", "tonA21", "glnV44", "lambda-", "hflA150(chr::Tn10)"]
},
{
label : "D1210",
parent : "HB101",
genotype : ["lacIq", "lacY+"]
},
{
label : "DB3.1",
purpose : "propagating plasmids with ccdB",
resistance : "streptomycin",
parent : "HB101",
genotype : ["F-", "gyrA462", "endA1", "glnV44", "D(sr1-recA)", "mcrB", "mrr", "hsdS20(rB-, mB-)", "ara14", "galK2", "lacY1", "proA2", "rpsL20(Smr)", "xyl5", "D(leu)", "mtl1"]
},
{
label : "DH1",
purpose : "cloning",
resistance : "nalidixic acid",
parent : "Hoffman-Berling 1100 strain derivative",
genotype : ["endA1", "recA1", "gyrA96", "thi-1", "glnV44", "relA1", "hsdR17(rK- mK+)", "lambda-"]
},
{
label : "DH5a",
purpose : "cloning",
resistance : "nalidixic acid",
parent : "DH1",
genotype : ["F-", "endA1", "glnV44", "thi-1", "recA1", "relA1", "gyrA96", "deoR", "nupG", "phi80dlacZD(M15)", "D(lacZYA-argF)U169", "hsdR17(rK- mK+)", "lambda-"]
},
]
};
- /
</script>
</html>
