Duffy:LIC Cloning - Revision history

Christopher C Vanlang: /* Contact */

Christopher C Vanlang — Fri, 30 Dec 2011 01:53:43 GMT

Contact

←Older revision		Revision as of 01:53, 30 December 2011
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	<!-- You can tag this protocol with various categories. See the [[Categories]] page for more information. -->		<!-- You can tag this protocol with various categories. See the [[Categories]] page for more information. -->
	[[Category:Protocol]]		[[Category:Protocol]]
-	~~[[Category:Needs attention]]~~
	[[Category:In vitro]]		[[Category:In vitro]]
	[[Category:DNA]]		[[Category:DNA]]
	[[Category:Escherichia coli]]		[[Category:Escherichia coli]]
-		+	[[Category:Cloning]]
	<!-- Move the relevant categories above this line to tag your protocol with the label		<!-- Move the relevant categories above this line to tag your protocol with the label
-
-
-
	[[Category:In vivo]]		[[Category:In vivo]]
-
	[[Category:In silico]]		[[Category:In silico]]
-
-
	[[Category:RNA]]		[[Category:RNA]]
-
	[[Category:Protein]]		[[Category:Protein]]
-
	[[Category:Chemical]]		[[Category:Chemical]]
-
-	~~[[Category:Cloning]]~~
	[[Category:Yeast]]		[[Category:Yeast]]
	-->		-->

Christopher C Vanlang at 01:44, 30 December 2011

Christopher C Vanlang — Fri, 30 Dec 2011 01:44:39 GMT

←Older revision		Revision as of 01:44, 30 December 2011
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	[[Category:Chemical]]		[[Category:Chemical]]

-		+	[[Category:Cloning]]
	[[Category:Yeast]]		[[Category:Yeast]]
	-->		-->

Jingyang Chen: /* References */

Jingyang Chen — Thu, 31 Jul 2008 03:21:55 GMT

References

←Older revision		Revision as of 03:21, 31 July 2008
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	<biblio>		<biblio>

-	#~~Bardóczy~~-~~BMC Biotechnol~~-2008 pmid=18371187	+	#Bardoczy-BMCBiotechnol-2008 pmid=18371187

	</biblio>		</biblio>

Jingyang Chen: /* References */

Jingyang Chen — Thu, 31 Jul 2008 03:21:17 GMT

References

←Older revision		Revision as of 03:21, 31 July 2008
Line 35:		Line 35:
	<biblio>		<biblio>

-	#pmid=18371187	+	#Bardóczy-BMC Biotechnol-2008 pmid=18371187

	</biblio>		</biblio>

Jingyang Chen: /* References */

Jingyang Chen — Thu, 31 Jul 2008 03:19:56 GMT

References

←Older revision		Revision as of 03:19, 31 July 2008
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	<!-- If this protocol has papers or books associated with it, list those references here. See the [[OpenWetWare:Biblio]] page for more information. -->		<!-- If this protocol has papers or books associated with it, list those references here. See the [[OpenWetWare:Biblio]] page for more information. -->
	<biblio>		<biblio>
		+
	#pmid=18371187		#pmid=18371187
		+
	</biblio>		</biblio>

Jingyang Chen: /* References */

Jingyang Chen — Thu, 31 Jul 2008 03:17:58 GMT

References

←Older revision		Revision as of 03:17, 31 July 2008
Line 34:		Line 34:
	<!-- If this protocol has papers or books associated with it, list those references here. See the [[OpenWetWare:Biblio]] page for more information. -->		<!-- If this protocol has papers or books associated with it, list those references here. See the [[OpenWetWare:Biblio]] page for more information. -->
	<biblio>		<biblio>
-	#~~Goldbeter-PNAS-1981~~ pmid=~~6947258~~	+	#pmid=18371187
-	~~#Jacob-JMB-1961 pmid=13718526~~	+
-	~~#Ptashne-Genetic-Switch isbn=0879697164~~	+
	</biblio>		</biblio>

Jingyang Chen: /* Overview */

Jingyang Chen — Thu, 31 Jul 2008 03:15:26 GMT

Overview

←Older revision		Revision as of 03:15, 31 July 2008
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-
	==Overview==		==Overview==

-	~~Replace this sentence with a brief description of the protocol and its goal~~.	+	Clone genes into pEU-E01-LIC1 protein expression vector using ligation independent cloning (LIC).

	==Materials==		==Materials==

Jingyang Chen: New page: ==Overview== Replace this sentence with a brief description of the protocol and its goal. ==Materials== List reagents, supplies and equipment necessary to perform the protocol here. Fo...

Jingyang Chen — Thu, 31 Jul 2008 03:14:24 GMT

New page: ==Overview== Replace this sentence with a brief description of the protocol and its goal. ==Materials== List reagents, supplies and equipment necessary to perform the protocol here. Fo...

New page

==Overview==

Replace this sentence with a brief description of the protocol and its goal.

==Materials==
List reagents, supplies and equipment necessary to perform the protocol here. For those materials which have their own OWW pages, link to that page. Alternatively, links to the suppliers' page on that material are also appropriate.

*supply 1 (i.e. tubes of a certain size? spreaders?)
*reagent 1
*X μL reagent 2
**component A (reagent 2 is made up of multiple components)
**component B
*equipment 1
*equipment 2

==Procedure==
#Step 1
#Step 2
#*Step 2 has some additional information that goes with it. i.e. Keep at 4°C.
#Step 3
##Step 3 has multiple sub-steps within it.
##Enumerate each of those.

==Notes==
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
#List troubleshooting tips here.
#You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
#Anecdotal observations that might be of use to others can also be posted here.

Please sign your name to your note by adding <font face="courier"><nowiki>'''*~~~~''':</nowiki></font> to the beginning of your tip.

==References==
'''Relevant papers and books'''

<biblio>
#Goldbeter-PNAS-1981 pmid=6947258
#Jacob-JMB-1961 pmid=13718526
#Ptashne-Genetic-Switch isbn=0879697164
</biblio>

==Contact==
*Who has experience with this protocol?

or instead, [[Talk:{{PAGENAME}}|discuss this protocol]].


[[Category:Protocol]]
[[Category:Needs attention]]
[[Category:In vitro]]
[[Category:DNA]]
[[Category:Escherichia coli]]