Drummond:Protocols: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Dadrummond (talk | contribs) (Akashi's test) |
|||
| Line 21: | Line 21: | ||
</div> | </div> | ||
{{Drummond_Bottom}} | {{Drummond_Bottom}} | ||
==Data Analysis Protocols== | |||
*[[Drummond:Akashi's Test|Akashi's test for translational accuracy selection]] | |||
Revision as of 08:44, 22 January 2009
Local Protocols
Here are some protocols we use in the lab.
- Extracting soluble and insoluble protein fractions
- Thoughts on lysis of yeast cells
- Resolving and detecting ubiquitin
- Using paromomycin to alter translation in yeast
- Cell preparation and protocol to view RFP/TFP fluorescence
- Using the Zeiss LSM 510 confocal microscope
- Yeast OD and cell viability model
- High Efficiency Yeast Transformation
- Yeast Genomic DNA Prep
- Isolating Total, Soluble and Insoluble Protein Fractions
- The relationship between the selection coefficient and relative growth rates: under construction
Remote Protocols
Rather rapid yeast genomic DNA prep (Gottschling Lab)
SDS-PAGE (Szostak Lab)
