Drummond:Protocols: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Dadrummond (talk | contribs) (reorg) |
Dadrummond (talk | contribs) |
||
| Line 15: | Line 15: | ||
==Remote Protocols== | ==Remote Protocols== | ||
*[http://www.fhcrc.org/science/labs/gottschling/yeast/qgprep.html Rather rapid yeast genomic DNA prep (Gottschling Lab)] | *[http://depts.washington.edu/younglab/yeastprotocols(htm)/sporeenrichment.htm Spore enrichment for random-spore analysis (from Rockmill Meth. Enz. 1991)] | ||
*[http://www.fhcrc.org/science/labs/gottschling/yeast/qgprep.html Rather rapid yeast genomic DNA prep (Gottschling Lab)] | |||
*[http://genetics.mgh.harvard.edu/szostakweb/resources/Public%20Protocols/protein_page/index.html SDS-PAGE (Szostak Lab)] | *[http://genetics.mgh.harvard.edu/szostakweb/resources/Public%20Protocols/protein_page/index.html SDS-PAGE (Szostak Lab)] | ||
Revision as of 15:42, 24 February 2009
Local Protocols
Here are some protocols we use in the lab.
- Resolving and detecting ubiquitin
- Using paromomycin to alter translation in yeast
- Cell preparation and protocol to view RFP/TFP fluorescence
- Using the Zeiss LSM 510 confocal microscope
- Yeast OD and cell viability model
- High-efficiency yeast transformation
- Yeast genomic DNA prep
- Isolating total, soluble and insoluble protein fractions
Remote Protocols
- Spore enrichment for random-spore analysis (from Rockmill Meth. Enz. 1991)
- Rather rapid yeast genomic DNA prep (Gottschling Lab)
- SDS-PAGE (Szostak Lab)
