Drummond:Protocols: Difference between revisions
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Here are some protocols we use in the lab. | Here are some protocols we use in the lab. | ||
*[[Drummond:Ubiquitin_Western|Resolving and detecting ubiquitin]] | *[[Drummond:Ubiquitin_Western|Resolving and detecting ubiquitin]] | ||
*[[Drummond:Paromomycin|Using paromomycin to alter translation in yeast]] | *[[Drummond:Paromomycin|Using paromomycin to alter translation in yeast]] | ||
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*[[Drummond:Confocal|Using the Zeiss LSM 510 confocal microscope]] | *[[Drummond:Confocal|Using the Zeiss LSM 510 confocal microscope]] | ||
*[[Drummond:Viability|Yeast OD and cell viability model]] | *[[Drummond:Viability|Yeast OD and cell viability model]] | ||
*[[Drummond:Transformation|High | *[[Drummond:Transformation|High-efficiency yeast transformation]] | ||
*[[Drummond:DNA Prep|Yeast | *[[Drummond:DNA Prep|Yeast genomic DNA prep]] | ||
*[[Drummond:Protein Isolation|Isolating | *[[Drummond:Protein Isolation|Isolating total, soluble and insoluble protein fractions]] | ||
*[[ | <!-- *[[Drummond:Solubility|Extracting soluble and insoluble protein fractions]] --> | ||
==Remote Protocols== | ==Remote Protocols== | ||
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==Data Analysis Protocols== | ==Data Analysis Protocols== | ||
*[[Drummond:Akashi's Test|Akashi's test for translational accuracy selection]] | *[[Drummond:Akashi's Test|Akashi's test for translational accuracy selection]] | ||
==Notes== | |||
*[[Drummond:PopGen|The relationship between the selection coefficient and relative growth rates: under construction]] | |||
*[[Drummond:Lysis|Thoughts on lysis of yeast cells]] | |||
</div> | </div> | ||
{{Drummond_Bottom}} | {{Drummond_Bottom}} |
Revision as of 15:09, 8 February 2009
Local Protocols
Here are some protocols we use in the lab.
- Resolving and detecting ubiquitin
- Using paromomycin to alter translation in yeast
- Cell preparation and protocol to view RFP/TFP fluorescence
- Using the Zeiss LSM 510 confocal microscope
- Yeast OD and cell viability model
- High-efficiency yeast transformation
- Yeast genomic DNA prep
- Isolating total, soluble and insoluble protein fractions