Drummond:Protocols: Difference between revisions
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*[[Drummond:Transformation|High Efficiency Yeast Transformation]] | *[[Drummond:Transformation|High Efficiency Yeast Transformation]] | ||
*[[Drummond:DNA Prep|Yeast Genomic DNA Prep]] | *[[Drummond:DNA Prep|Yeast Genomic DNA Prep]] | ||
*[[Drummond:Protein Isolation|Isolating Total, Soluble and Insoluble Protein Fractions]] | |||
*[[The relationship between the selection coefficient and relative growth rates: under construction]] | *[[The relationship between the selection coefficient and relative growth rates: under construction]] | ||
Revision as of 13:05, 25 November 2008
Local Protocols
Here are some protocols we use in the lab.
- Extracting soluble and insoluble protein fractions
- Thoughts on lysis of yeast cells
- Resolving and detecting ubiquitin
- Using paromomycin to alter translation in yeast
- Cell preparation and protocol to view RFP/TFP fluorescence
- Using the Zeiss LSM 510 confocal microscope
- Yeast OD and cell viability model
- High Efficiency Yeast Transformation
- Yeast Genomic DNA Prep
- Isolating Total, Soluble and Insoluble Protein Fractions
- The relationship between the selection coefficient and relative growth rates: under construction
Remote Protocols
Rather rapid yeast genomic DNA prep (Gottschling Lab)
SDS-PAGE (Szostak Lab)
