Drummond:Lysis: Difference between revisions
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This is used for lysing yeast at pH6.8. For pH8 lyses, use the variation of SUME with Tris buffer known as SUTE. | This is used for lysing yeast at pH6.8. For pH8 lyses, use the variation of SUME with Tris buffer known as SUTE. | ||
from [http://www.fhcrc.org/science/labs/gottschling/yeast/yeastip.html?&&printfriendly=yes Yeast Protocols] | |||
Revision as of 11:26, 21 June 2007
SUME Buffer (1% SDS, 8M Urea, 10mM MOPS, pH 6.8, 10mM EDTA) To make 100ml:
* 1.0ml 1M MOPS, pH6.8 stock solution * 1.0g SDS, or 10ml 10% SDS stock solution * 48.05g Urea * 2.0ml 0.5M EDTA stock solution
This is used for lysing yeast at pH6.8. For pH8 lyses, use the variation of SUME with Tris buffer known as SUTE.
from Yeast Protocols
