Drummond:Coupling: Difference between revisions
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The parameters can then be fit by | The parameters can then be fit by maximizing the log-likelihood function. The whole process is termed <em>logistic regression</em>. | ||
==Application to WW domains== | |||
Given only the Socolich et al. data, we can estimate the probability of folding given mean pair probability, max % identity, # of missed pairs. First question: what's the best predictor? | |||
:<math> | :<math> |
Revision as of 19:32, 13 July 2008
Prediction of probability of protein folding
Assume that folding is a binary outcome represented by the random variable [math]\displaystyle{ F }[/math]. Given some predictor [math]\displaystyle{ X }[/math] (such as mean pair probability, max % identity, # of missed pairs), we want to infer [math]\displaystyle{ Pr(F|X) }[/math]. We assume that there is a sigmoidal relationship between X and the probability of folding,
- [math]\displaystyle{ \Pr(F|X) = p = 1/(1 + e^{aX + b}) }[/math]
where [math]\displaystyle{ a }[/math] and [math]\displaystyle{ b }[/math] quantify the steepness and position of the step function. If We can write down a likelihood of the observed data, where [math]\displaystyle{ x_i }[/math] is the value of the predictor [math]\displaystyle{ X }[/math] for an actual protein
[math]\displaystyle{ L(F|\{x_i\})\! }[/math] [math]\displaystyle{ = \prod_{i \in \textrm{folded}} \Pr(F|X=x_i) \prod_{j \in \textrm{unfolded}} (1 - \Pr(F|X=x_j)) }[/math] [math]\displaystyle{ \ln L(F|\{x_i\})\! }[/math] [math]\displaystyle{ = \sum_{i \in \textrm{folded}} \ln \Pr(F|X=x_i) \prod_{j \in \textrm{unfolded}} \ln (1 - \Pr(F|X=x_j)) }[/math] [math]\displaystyle{ \ln L(F|\{x_i\})\! }[/math] [math]\displaystyle{ = -\sum_{i \in \textrm{all}} \ln (1 + e^{ax_i + b}) + \sum_{j \in \textrm{unfolded}} a x_j + b }[/math]
The parameters can then be fit by maximizing the log-likelihood function. The whole process is termed logistic regression.
Application to WW domains
Given only the Socolich et al. data, we can estimate the probability of folding given mean pair probability, max % identity, # of missed pairs. First question: what's the best predictor?
- [math]\displaystyle{ Ax + b = \log \frac{1-p}{p} \! }[/math]
can be fit by maximum likelihood.