Drummond:Confocal: Difference between revisions
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# Turn on mercury lamp | ==Starting up the system== | ||
# Turn on main power | # Turn on mercury lamp (after 1/2 hour, turn down to 50% power) | ||
# Turn on main power | |||
# Turn on computer | # Turn on computer | ||
# Turn on lasers | # Turn on lasers | ||
# Open LSM510 software | # Open LSM510 software | ||
# Set laser output: | |||
#* Helium-neon 1: 50% (543nm line suitable for RFP excitation) | |||
#* Argon: 5% (458nm line suitable for TFP excitation) | |||
==Slide preparation== | ==Slide preparation== | ||
(cf. [[Fluorescence|cell preparation]] | (cf. [[Drummond:Fluorescence|cell preparation]]) | ||
* Prepare 15 mins in advance to allow drying | * Prepare 15 mins in advance to allow drying | ||
* 15uL droplet | * 15uL droplet | ||
* No. 1 1/2 cover slip | * No. 1 1/2 cover slip | ||
* Push down gently on cover slip to squish out extra liquid and any air bubbles. Cover slip should not float! | * Push down gently on cover slip to squish out extra liquid and any air bubbles. Cover slip should not float! | ||
Revision as of 14:18, 26 June 2008
Starting up the system
- Turn on mercury lamp (after 1/2 hour, turn down to 50% power)
- Turn on main power
- Turn on computer
- Turn on lasers
- Open LSM510 software
- Set laser output:
- Helium-neon 1: 50% (543nm line suitable for RFP excitation)
- Argon: 5% (458nm line suitable for TFP excitation)
Slide preparation
(cf. cell preparation)
- Prepare 15 mins in advance to allow drying
- 15uL droplet
- No. 1 1/2 cover slip
- Push down gently on cover slip to squish out extra liquid and any air bubbles. Cover slip should not float!
