DpnI

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*Incubate at 37°C.
*Incubate at 37°C.
*Heat inactivate by incubating at 80°C for 20 minutes.
*Heat inactivate by incubating at 80°C for 20 minutes.
 +
*DpnI cleaves '''hemi'''-adenomethylated dam sites 60X more slowly than fully methylated. [http://www.neb.com/nebecomm/products/faqproductR0176.asp#10 NEB FAQ]
===Relevant protocols===
===Relevant protocols===

Revision as of 11:10, 28 March 2008

Contents

Purpose

  • To digest methylated GATC sites. Useful for removing cell-derived plasmid template from PCR samples.

Procurement

  • Can be ordered from NEB.
  • Typically stored at -20°C.

Use

  • Use in NEBuffer 4. However, can be added directly to PCR sample.
  • Incubate at 37°C.
  • Heat inactivate by incubating at 80°C for 20 minutes.
  • DpnI cleaves hemi-adenomethylated dam sites 60X more slowly than fully methylated. NEB FAQ

Relevant protocols

Safety

  • None known.

References

Contact

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