DpnI: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 22: | Line 22: | ||
==Contact== | ==Contact== | ||
*[[Reshma Shetty]] | *[[Reshma Shetty]] | ||
[[Category:Material]] [[Category:Enzyme]] [[Category:Endonuclease]] | |||
Revision as of 10:05, 27 May 2007
Purpose
- To digest methylated GATC sites. Useful for removing cell-derived plasmid template from PCR samples.
Procurement
- Can be ordered from NEB.
- Typically stored at -20°C.
Use
- Use in NEBuffer 4. However, can be added directly to PCR sample.
- Incubate at 37°C.
- Heat inactivate by incubating at 80°C for 20 minutes.
Relevant protocols
Safety
- None known.
