Disrupting the biofilm: Difference between revisions
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(New page: The crystal violet assay is great to quantify biofilm formation quickly in a high-throughput way for single- and multiple-strain biofilms. Nevertheless, we want to look at the strain compo...) |
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Revision as of 12:39, 12 October 2007
The crystal violet assay is great to quantify biofilm formation quickly in a high-throughput way for single- and multiple-strain biofilms. Nevertheless, we want to look at the strain composition in each biofilm in a high. For this, we need to disrupt the biofilm back into suspended cells so that we can quantify their relative amounts through fluorescence.
Biofilm disruption solution
- 1% SDS
- 5 mM EDTA
- 100 mM NaCl
- 50 mM Tris
- pH 7.4
