Determined Concentrations of Labeled Oligos: Difference between revisions
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Manuel Hora (talk | contribs) (Autocreate 2011/08/25 Entry for Biomod/2011/TUM/TNT:Notebook/Biomod/2011/TUM/TNT) |
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== | == determined concentrations of labeled oligos == | ||
* absorption coefficients of unlabeled oligos were determined with a dilution series | |||
* concentrations of labeled oligos were determined using these absorption coefficients at 260nm | |||
<br> | |||
* results: | |||
* the_U_175_Atto550_ddCTP: 6.7µM | |||
* the_U_183_Atto550_ddCTP: 1.1µM | |||
* the_U_197_Atto550_ddCTP: 2.6µM | |||
* the_U_204_Atto647N_ddUTP: 3.9µM | |||
* the_U_218_Atto647N_ddUTP: 1.3µM | |||
* the_U_204_Atto647N_ddUTP: 2.6µM | |||
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Revision as of 07:31, 25 August 2011
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determined concentrations of labeled oligos
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