Davidson:Plasmids and Bacteria

• Transform bacteria We are using Salmonella typhimurium for the Hin protein as it was the referenced species in all of the literature.
• Create RE biobrick (completed)
• Isolating genomic DNA from Salmonella typhimurium (completed)
• Ordered oligos for recombinational enhancer and primers for the hin gene
  • These sequences will be transformed into RFP and possibly GFP plasmids
• Performed Minipreps to isolate RFP and GFP plasmids to be used for transformations.