Dandekar & Chandler:Pyocyanin Extraction
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Pyocyanin Extraction
Pyocyanin [1] is a secondary metabolite secreted by the Gram negative bacterium Pseudomonas aeruginosa[2]. This protocol was established to quantify the pyocyanin produced by a P. aeruginosa culture in broth media.
Principle
Pyocyanin is an extracellular redox-active phenazine that contains a distinctive blue-green pigment. This pigmented compound can be isolated from cellular contaminants and quantified with a spectrophotometer.
Materials Needed
- Spectrophotometer capable of reading 520 nm
- Vortex
- Sterile Cuvets
- Nanopure H20
- 10mL test tube
- 0.2m HCl
- Chloroform
- 1.5ml eppendorf tubes
