Dandekar & Chandler:Pyocyanin Extraction: Difference between revisions
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===Principle=== | ===Principle=== | ||
Pyocyanin contains a | Pyocyanin is an extracellular redox-active phenazine that contains a distinctive blue-green pigment. This pigmented compound can be isolated from cellular contaminants and quantified with a spectrophotometer. | ||
===Materials Needed=== | ===Materials Needed=== | ||
===Protocol=== | ===Protocol=== | ||
Revision as of 10:42, 9 October 2013
Pyocyanin Extraction
Pyocyanin [1] is a secondary metabolite secreted by the Gram negative bacterium Pseudomonas aeruginosa[2]. This protocol was established to quantify the pyocyanin produced by a P. aeruginosa culture in broth media.
Principle
Pyocyanin is an extracellular redox-active phenazine that contains a distinctive blue-green pigment. This pigmented compound can be isolated from cellular contaminants and quantified with a spectrophotometer.
