http://openwetware.org/index.php?title=DYT&action=history Revision history for this page on the wiki en MediaWiki 1.13.2 Tue, 21 May 2013 14:25:43 GMT http://openwetware.org/index.php?title=DYT&diff=573699&oldid=prev <p>New page: 1.7- DYT medium For 500 mL * 8.0 g tryptone * 5.0 g yeast extract * 2.5 g NaCl For 100 mL * 1.6 g tryptone * 1.0 g yeast extract * 0.5 g NaCl *mix in DW *add 10 N NaOH (about 2.5 drops...</p> <p><b>New page</b></p><div>1.7- DYT medium<br /> For 500 mL<br /> * 8.0 g tryptone <br /> * 5.0 g yeast extract<br /> * 2.5 g NaCl<br /> <br /> For 100 mL<br /> * 1.6 g tryptone <br /> * 1.0 g yeast extract<br /> * 0.5 g NaCl<br /> <br /> *mix in DW<br /> *add 10 N NaOH (about 2.5 drops for 100 ml)<br /> *check that pH=7.0 with pH paper; adjust as needed<br /> *aliquot: add 3 ml to 15 ml glass tubes, cap loosely<br /> *autoclave in a rack in a tub with 4 cm of water<br /> *store at 4°C<br /> <br /> [[Category:Material]] [[Category:Standard Media]]</div> Fri, 23 Dec 2011 21:33:14 GMT Christopher C Vanlang http://openwetware.org/wiki/Talk:DYT