DOs and DONTs of Good Lab Citizenship: Difference between revisions
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* Stay SAFE! | * Stay SAFE! | ||
* Be CONSIDERATE of others (their stuff, needs, space, etc) | * Be CONSIDERATE of others (their stuff, needs, space, etc) | ||
* Always ASK SOMEONE if you’re not sure about something. | * Always ASK SOMEONE if you’re not sure about something. First ask the iGEM graduate advisors before asking other members of the Endy lab. | ||
* THINK before you do. | * THINK before you do. | ||
* CLEAN UP after yourself. | * CLEAN UP after yourself, both on a day-to-day basis, and once the competition is over. If you’ve caused a spill in a centrifuge, clean it up. | ||
* Maintain a CLEAN workspace. | * Maintain a CLEAN workspace. | ||
* REFILL the stocks of pipette tip boxes above the PCR machines if you are taking the penultimate or the last box of a kind (e.g. 1000ul tips) | |||
* If we’re out of anything (e.g. gloves, cuvettes), ASK your graduate leaders on how to reorder. | |||
* Maintain STERILE technique. | * Maintain STERILE technique. | ||
* ASK someone before you move their stuff. | * ASK someone before you move their stuff. | ||
* BALANCE the | * Before putting a container into the metal tray for cleaning/sterilizing, REMOVE any labels, BLEACH (if biological material) and RINSE the container. | ||
* BALANCE the centrifuges. | |||
* ASK SOMEONE how to use a piece of equipment if you haven’t used it before. | * ASK SOMEONE how to use a piece of equipment if you haven’t used it before. | ||
* If you use the | * If you use the roller drums or shakers in the 30C or 37C rooms, remember to TURN THEM BACK ON. | ||
* CLOSE refrigerator doors!! | * CLOSE refrigerator doors!! | ||
* Use YOUR OWN | * Use YOUR OWN master stocks of chemicals and supplies; we’ll tell you which other supplies you’re allowed to use. | ||
* READ | * READ PROTOCOLS before you attempt them. | ||
* Keep any proteins ( | * Keep any proteins (endonucleases, ligase, Taq supermix) always ON ICE | ||
* CHANGE TIPS between aliquots of important stocks. | * CHANGE TIPS between aliquots of important stocks. | ||
* WEAR GLOVES when working w/ dangerous/sensitive chemicals ( | * WEAR GLOVES when working w/ dangerous/sensitive chemicals (phenol, RNA, etc). | ||
* Keep a good | * Keep a good LAB NOTEBOOK so you know what you did. | ||
* LABEL TUBES | * LABEL TUBES | ||
* Store | * Store GLYCEROLS appropriately | ||
* If you did something wrong, CONFESS and tell someone. You won’t get in trouble for accidents. | * If you did something wrong, CONFESS and tell someone. You won’t get in trouble for accidents, but you’ll get in trouble for not letting people know. | ||
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* Don’t EVER COMPROMISE SAFETY! | * Don’t EVER COMPROMISE SAFETY! | ||
* Don’t Be too shy to ASK QUESTIONS | * Don’t Be too shy to ASK QUESTIONS. | ||
* Don’t Start something without THINKING about what you’re doing | * Don’t Start something without THINKING about what you’re doing | ||
* Don’t Use a MACHINE without having received training on it. | * Don’t Use a MACHINE without having received training on it. | ||
* Don’t POUR hazardous or unknown chemicals down the sink. | * Don’t POUR hazardous or unknown chemicals down the sink. What can be poured down the sink is written on a sheet next to the sink. | ||
* Don’t | * Don’t pour MOLTEN AGAR down the sink as this clog the pipes below the sink. | ||
* Don’t make a MESS of communal space such as refrigerators | |||
* Don’t put SHARPS in the Biowaste, because they’ll make holes in the plastic, causing molten agar to leak through when autoclaving | |||
* Don’t autoclave in the “kitchen”, during the kitchen working hours (~6am-2pm). Do not leave autoclaved material in the kitchen autoclaved overnight. | |||
* Don’t leave a door to a cold or warm room OPEN. | |||
* Don’t IGNORE equipment alarms | * Don’t IGNORE equipment alarms | ||
* Don’t USE master stocks other than your own without permission. | * Don’t USE master stocks other than your own without permission, especially not lab ANTIBIOTICS. | ||
* Don’t | * Do not hold on to FLASKS or bottles for long after your’re done with them. Instead, when you are done with flasks/bottles, BLEACH them (if you used them to grow cultures) and put them in the metal tray near the sink so they can go back into circulation. | ||
* Don’t just DUMP your biohazard waste into other people’s waste bins and then not clean it up. | |||
* Don’t leave your stuff lying around in other people’s areas. | |||
* Don’t EAT/DRINK in lab (go to the tea room to eat lunch and hang out). | * Don’t EAT/DRINK in lab (go to the tea room to eat lunch and hang out). | ||
* Don’t PLAY | * Don’t PLAY MUSIC out loud (not everyone likes your taste). | ||
* Don’t DATE people you work with.* | * Don’t DATE people you work with.* | ||
Revision as of 13:06, 10 June 2008
DO's
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DON'Ts
* May occasionally result in marriage. |