DNA separation in different agarose gels

This experiment was aimed to show the different band separation in 1%, 2% and 3% DNA agarose gels. 1 kbp and 100 bp DNA ladders were used and run at the same time for 20 minutes at 100 Volts (~400 mA). The distance of bands traveling in the gels were compared. Ladder bands, especially the larger DNA molecules, were shifting towards the top of the gel as agarose concentration was increased.

1 kbp and 100 bp in illuminated DNA agarose gels cross sections

File:DNA Gel agarose cross sections.jpg

Figure 1. Bands separation in 1 kbp and 100 bps DNA ladder are shown in different agarose gel concentration. Gels are illuminated under UV light condition shortly after 20 minutes running time.

1 kbp in different agarose gels

Figure 2. 1 kbp DNA ladder (NEB, 250 ng) bands separation in different agarose gels concentration

1kbp DNA ladder by NEB

100 bp in different agarose gels

File:100 bps DNA ladder.jpg

Figure 3. 100 bps DNA ladder (NEB, 500 ng) bands separation in different agarose gels concentration

See also

• Agarose gel electrophoresis