DNA ladder

Prepared DNA ladder

• NEB's 2-log ladder is a good choice for most samples.
• Deionized Water
• Loading dye

Mix NEB 2-log ladder with deionized water to a 100 ng/μL dilution. Add loading dye and store at 4°C. Load 12 μL per lane for 1 μg of DNA.

To make 1.2 mL prepared ladder, combine:

1. 900μL deionized water
2. 100μL 2-log ladder (at 1μg/μL)
3. 200μL loading dye