DNA ladder
From OpenWetWare
(Difference between revisions)
Current revision (11:34, 11 March 2008) (view source) (add tags) |
|||
| Line 11: | Line 11: | ||
#100μL 2-log ladder (at 1μg/μL) | #100μL 2-log ladder (at 1μg/μL) | ||
#200μL loading dye | #200μL loading dye | ||
| + | |||
| + | |||
| + | [[Category:Material]] [[Category:Agarose gel electrophoresis]] | ||
Current revision
Prepared DNA ladder
- NEB's 2-log ladder is a good choice for most samples.
- Deionized Water
- Loading dye
Mix NEB 2-log ladder with deionized water to a 100 ng/μL dilution. Add loading dye and store at 4°C. Load 12 μL per lane for 1 μg of DNA.
To make 1.2 mL prepared ladder, combine:
- 900μL deionized water
- 100μL 2-log ladder (at 1μg/μL)
- 200μL loading dye
