DNA ladder

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*[http://www.neb.com/ NEB]'s [http://www.neb.com/nebecomm/products/productN3200.asp 2-log ladder] is a good choice for most samples.
*[http://www.neb.com/ NEB]'s [http://www.neb.com/nebecomm/products/productN3200.asp 2-log ladder] is a good choice for most samples.
*Deionized Water
*Deionized Water
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*Loading dye (see above)
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*[[Loading dye]]
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Mix NEB 2-log ladder with deionized water to a 100 ng/μL dilution.  Add [[Loading dye | loading dye]] and store at 4°C.  Load 12 μL per lane for 1 μg of DNA.
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Mix NEB 2-log ladder with deionized water to a 100 ng/μL dilution.  Add loading dye and store at 4°C.  Load 12 μL per lane for 1 μg of DNA.
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To make 1.2 mL prepared ladder, combine:
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#900μL deionized water
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#100μL 2-log ladder (at 1μg/μL)
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#200μL loading dye
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[[Category:Material]] [[Category:Agarose gel electrophoresis]]

Current revision

Prepared DNA ladder

Mix NEB 2-log ladder with deionized water to a 100 ng/μL dilution. Add loading dye and store at 4°C. Load 12 μL per lane for 1 μg of DNA.

To make 1.2 mL prepared ladder, combine:

  1. 900μL deionized water
  2. 100μL 2-log ladder (at 1μg/μL)
  3. 200μL loading dye
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