DNA extraction from tissue - Revision history

Vaishnavi Ananth: /* BioStream version */

Vaishnavi Ananth — Thu, 19 Nov 2009 09:45:48 GMT

BioStream version

←Older revision		Revision as of 09:45, 19 November 2009
Line 37:		Line 37:
	#Resuspend pellet in MilliQ water		#Resuspend pellet in MilliQ water

-	==~~BioStream~~ version==	+	==BioCoder version==
-	Following is the DNA extraction from tissue protocol in ~~BioStream~~, a high-level programming language for expressing biology protocols. What you see here is the auto-generated text ouput of the protocol that was coded up in ~~BioStream~~ (see Source code). More information about ~~BioStream~~ can be found on my home page. Feel free to mail me your comments/ suggestions.[[User:Vaishnavi Ananth\|Vaishnavi]]	+	Following is the DNA extraction from tissue protocol in BioCoder, a high-level programming language for expressing biology protocols. What you see here is the auto-generated text ouput of the protocol that was coded up in BioCoder (see Source code). More information about BioCoder can be found on my home page. Feel free to mail me your comments/ suggestions.[[User:Vaishnavi Ananth\|Vaishnavi]]
	====Text Output====		====Text Output====
	[[DNA extraction from tissue protocol]]		[[DNA extraction from tissue protocol]]

Vaishnavi Ananth at 08:37, 29 September 2009

Vaishnavi Ananth — Tue, 29 Sep 2009 08:37:19 GMT

←Older revision		Revision as of 08:37, 29 September 2009
Line 36:		Line 36:
	#Airdry pellet		#Airdry pellet
	#Resuspend pellet in MilliQ water		#Resuspend pellet in MilliQ water
		+
		+	==BioStream version==
		+	Following is the DNA extraction from tissue protocol in BioStream, a high-level programming language for expressing biology protocols. What you see here is the auto-generated text ouput of the protocol that was coded up in BioStream (see Source code). More information about BioStream can be found on my home page. Feel free to mail me your comments/ suggestions.[[User:Vaishnavi Ananth\|Vaishnavi]]
		+	====Text Output====
		+	[[DNA extraction from tissue protocol]]
		+	====Source Code====
		+	[[DNA extraction from tissue protocol - source code]]

	== See also ==		== See also ==

Marcus McHale: /* See also */

Marcus McHale — Fri, 20 Jun 2008 01:30:41 GMT

Marcus McHale: /* See also */

Marcus McHale — Wed, 23 Apr 2008 06:48:40 GMT

Jakob Suckale: categories

Jakob Suckale — Wed, 16 Jan 2008 15:22:03 GMT

Jakob Suckale at 15:20, 16 January 2008

Jakob Suckale — Wed, 16 Jan 2008 15:20:48 GMT

←Older revision		Revision as of 15:20, 16 January 2008
Line 3:		Line 3:
	Protocol for extraction of DNA from tissue or embryos.		Protocol for extraction of DNA from tissue or embryos.

-	==~~ProK Digestion~~==	+	== Proteinase K digestion ==
	#Mix DNA extraction buffer		#Mix DNA extraction buffer
	#*98 μl [[Oneill_Lab:Chemicals#ReagentB\|ReagentB]]		#*98 μl [[Oneill_Lab:Chemicals#ReagentB\|ReagentB]]
Line 11:		Line 11:
	#Incubate at 50°C overnight		#Incubate at 50°C overnight

-	==PCI==	+	== Phenol/chloroform/isoamyl (PCI) ==
	#Prepare PCI mix		#Prepare PCI mix
	##One part tris-saturated phenol to one part 24:1 Chloroform:Isoamyl alcohol		##One part tris-saturated phenol to one part 24:1 Chloroform:Isoamyl alcohol
Line 26:		Line 26:
	#Continue to precipitation		#Continue to precipitation

-	==Ethanol ~~Precipitation~~==	+	== Ethanol precipitation ==
	#Add 2 volumes 100% EtOH		#Add 2 volumes 100% EtOH
	#Add 1/10 volume 3M Sodium Acetate pH 5.0		#Add 1/10 volume 3M Sodium Acetate pH 5.0
Line 46:		Line 46:
	* [[Image:2stars.png]] [http://www.bio.com/protocolstools/protocol.jhtml?id=p1689 DNA extraction from tail or tissue] - at BioProtocol from unknown contributor		* [[Image:2stars.png]] [http://www.bio.com/protocolstools/protocol.jhtml?id=p1689 DNA extraction from tail or tissue] - at BioProtocol from unknown contributor
	* [http://www.protocol-online.org/prot/Molecular_Biology/DNA/DNA_Extraction___Purification/DNA_Extraction_from_Cell_and_Tissue/ Protocol Online links to DNA extraction protocols]		* [http://www.protocol-online.org/prot/Molecular_Biology/DNA/DNA_Extraction___Purification/DNA_Extraction_from_Cell_and_Tissue/ Protocol Online links to DNA extraction protocols]
-	*

Jakob Suckale: backlink

Jakob Suckale — Wed, 16 Jan 2008 15:14:56 GMT

backlink

←Older revision		Revision as of 15:14, 16 January 2008
Line 1:		Line 1:
		+	{{back to protocols}}
		+
	Protocol for extraction of DNA from tissue or embryos.		Protocol for extraction of DNA from tissue or embryos.

Jakob Suckale: external links

Jakob Suckale — Wed, 16 Jan 2008 15:12:57 GMT

external links

←Older revision		Revision as of 15:12, 16 January 2008
Line 37:		Line 37:
	== See also ==		== See also ==
	* [[Oneill Lab:DNA Extraction]]		* [[Oneill Lab:DNA Extraction]]
		+	* [[Mouse tissue lysis for genotyping]]
		+
		+	== External links ==
		+	* [[Image:3stars.png]] [http://www.riedlab.nci.nih.gov/publications/2439.2451_DNA_Prep_Fresh_Froz_Tiss.pdf DNA from fresh or frozen tissue by Ried lab]
		+	* [[Image:3stars.png]] [http://www.protocol-online.org/cgi-bin/prot/view_cache.cgi?ID=1178 DNA from liver by Bowtell and colleagues] - cached version from protocol online
		+	* [[Image:2stars.png]] [http://www.bio.com/protocolstools/protocol.jhtml?id=p1689 DNA extraction from tail or tissue] - at BioProtocol from unknown contributor
		+	* [http://www.protocol-online.org/prot/Molecular_Biology/DNA/DNA_Extraction___Purification/DNA_Extraction_from_Cell_and_Tissue/ Protocol Online links to DNA extraction protocols]
		+	*

Jakob Suckale: see also

Jakob Suckale — Wed, 16 Jan 2008 14:52:11 GMT

Jakob Suckale: * page created * based on O'Neill lab protocol

Jakob Suckale — Wed, 16 Jan 2008 14:46:05 GMT

*** page created *** based on O'Neill lab protocol

New page

Protocol for extraction of DNA from tissue or embryos.

==ProK Digestion==
#Mix DNA extraction buffer
#*98 μl [[Oneill_Lab:Chemicals#ReagentB|ReagentB]]
#*2 μl ProteinaseK
#*Mix fresh. 100 μl is enough for a small pea size chunk of tissue or one embryo
#Place small piece of tissue or embryo into a microfuge tube containing 100 μl of extraction buffer
#Incubate at 50°C overnight

==PCI==
#Prepare PCI mix
##One part tris-saturated phenol to one part 24:1 Chloroform:Isoamyl alcohol
##Shake thoroughly to make emulsion
#Add one volume of PCI to extracted sample
#Shake tubes for 10 seconds
#Centrifuge at max speed for 5 minutes
#Remove aqueous phase to a new tube
#Repeat as needed
#Add one volume 24:1 chloroform:isoamyl alcohol
#Shake tubes for 10 seconds
#Centrifuge at max speed for 5 minutes
#Remove aqueous phase to a new tube
#Continue to precipitation

==Ethanol Precipitation==
#Add 2 volumes 100% EtOH
#Add 1/10 volume 3M Sodium Acetate pH 5.0
#Centrifuge at max speed for 10 minutes
#Decant ethanol
#Add 150 μl 70% EtOH
#Centrifuge at max speed for 2 minutes
#Pipette out ethanol
#Airdry pellet
#Resuspend pellet in MilliQ water

←Older revision		Revision as of 01:30, 20 June 2008
Line 40:		Line 40:
	* [[Oneill Lab:DNA Extraction]]		* [[Oneill Lab:DNA Extraction]]
	* [[Mouse tissue lysis for genotyping]]		* [[Mouse tissue lysis for genotyping]]
-	* [[DNA ~~Extraction~~ - Salting Out]]	+	* [[DNA extraction - Salting Out]]

	== External links ==		== External links ==

←Older revision		Revision as of 06:48, 23 April 2008
Line 40:		Line 40:
	* [[Oneill Lab:DNA Extraction]]		* [[Oneill Lab:DNA Extraction]]
	* [[Mouse tissue lysis for genotyping]]		* [[Mouse tissue lysis for genotyping]]
		+	* [[DNA Extraction - Salting Out]]

	== External links ==		== External links ==

←Older revision		Revision as of 15:22, 16 January 2008
Line 46:		Line 46:
	* [[Image:2stars.png]] [http://www.bio.com/protocolstools/protocol.jhtml?id=p1689 DNA extraction from tail or tissue] - at BioProtocol from unknown contributor		* [[Image:2stars.png]] [http://www.bio.com/protocolstools/protocol.jhtml?id=p1689 DNA extraction from tail or tissue] - at BioProtocol from unknown contributor
	* [http://www.protocol-online.org/prot/Molecular_Biology/DNA/DNA_Extraction___Purification/DNA_Extraction_from_Cell_and_Tissue/ Protocol Online links to DNA extraction protocols]		* [http://www.protocol-online.org/prot/Molecular_Biology/DNA/DNA_Extraction___Purification/DNA_Extraction_from_Cell_and_Tissue/ Protocol Online links to DNA extraction protocols]
		+
		+
		+	[[Category:Protocol]]
		+	[[Category:In vivo]]
		+	[[Category:Mouse]]
		+	[[Category:DNA]]

←Older revision		Revision as of 14:52, 16 January 2008
Line 34:		Line 34:
	#Airdry pellet		#Airdry pellet
	#Resuspend pellet in MilliQ water		#Resuspend pellet in MilliQ water
		+
		+	== See also ==
		+	* [[Oneill Lab:DNA Extraction]]

DNA extraction from tissue - Revision history

Vaishnavi Ananth: /* BioStream version */

Vaishnavi Ananth at 08:37, 29 September 2009

Marcus McHale: /* See also */

Marcus McHale: /* See also */

Jakob Suckale: categories

Jakob Suckale at 15:20, 16 January 2008

Jakob Suckale: backlink

Jakob Suckale: external links

Jakob Suckale: see also

Jakob Suckale: *** page created *** based on O'Neill lab protocol

Jakob Suckale: * page created * based on O'Neill lab protocol