DNA dyes: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 2: | Line 2: | ||
== Dyes for DNA in agarose gel electrophoresis == | == Dyes for DNA in agarose gel electrophoresis == | ||
* [[ethidium bromide]] - traditional DNA stain | * [[ethidium bromide]] - traditional DNA stain, quantum yield 0.2 | ||
* [[SYBR Gold]] - DNA & RNA, 10x more sensitive than EtBr | * [[SYBR Gold]] - DNA & RNA, 10x more sensitive than EtBr, quantum yield 0.6, 500µl 10'000x €165 [http://www.lifetechnologies.com/order/catalog/product/S11494 S-11494] | ||
* [[SYBR Green I]] - better for DNA, 20x more sensitive than EtBr (60 pg per band with 300 nm transillumination) | * [[SYBR Green I]] - better for DNA, 20x more sensitive than EtBr (60 pg per band with 300 nm transillumination), quantum yield 0.8 | ||
* [[SYBR Green II]] - better for RNA, detection limit is 500 pg of RNA per band in non-denaturing gels with 300 nm transillumination | * [[SYBR Green II]] - better for RNA, detection limit is 500 pg of RNA per band in non-denaturing gels with 300 nm transillumination, quantum yield 0.5 | ||
== Dyes for DNA in fluorescent microscopy == | == Dyes for DNA in fluorescent microscopy == | ||
| Line 20: | Line 20: | ||
* [http://en.wikipedia.org/wiki/Ethidium_bromide ethidium bromide], [http://en.wikipedia.org/wiki/SYBR_green SYBR Green 1], [http://en.wikipedia.org/wiki/SYBR_safe SYBR safe] | * [http://en.wikipedia.org/wiki/Ethidium_bromide ethidium bromide], [http://en.wikipedia.org/wiki/SYBR_green SYBR Green 1], [http://en.wikipedia.org/wiki/SYBR_safe SYBR safe] | ||
* [http://en.wikipedia.org/wiki/DAPI DAPI], [http://en.wikipedia.org/wiki/Hoechst_stain Hoechst], [http://en.wikipedia.org/wiki/Propidium_iodide propidium iodide], [http://en.wikipedia.org/wiki/YOYO-1 YOYO 1] | * [http://en.wikipedia.org/wiki/DAPI DAPI], [http://en.wikipedia.org/wiki/Hoechst_stain Hoechst], [http://en.wikipedia.org/wiki/Propidium_iodide propidium iodide], [http://en.wikipedia.org/wiki/YOYO-1 YOYO 1] | ||
* [http://www.lifetechnologies.com/de/de/home/references/molecular-probes-the-handbook/nucleic-acid-detection-and-genomics-technology/nucleic-acid-detection-and-quantitation-in-electrophoretic-gels-and-capillaries.html Life technology page on nucleic acid detection] | |||
Revision as of 06:28, 13 June 2014
DNA dyes stain deoxyribonucleic acid for laboratory purposes such as detection and quantification. Many DNA dyes also bind to RNA and could be more broadly described as nucleic acid stains. Common dyes included ethidium bromide (EtBr), esp. for agarose gel electrophoresis of DNA, and DAPI for staining the cell nucleus in fluorescent microscopy.
Dyes for DNA in agarose gel electrophoresis
- ethidium bromide - traditional DNA stain, quantum yield 0.2
- SYBR Gold - DNA & RNA, 10x more sensitive than EtBr, quantum yield 0.6, 500µl 10'000x €165 S-11494
- SYBR Green I - better for DNA, 20x more sensitive than EtBr (60 pg per band with 300 nm transillumination), quantum yield 0.8
- SYBR Green II - better for RNA, detection limit is 500 pg of RNA per band in non-denaturing gels with 300 nm transillumination, quantum yield 0.5
Dyes for DNA in fluorescent microscopy
Binding mode
- intercalating: EtBr
See also
Wikipedia entries for:
