DNA blot (Southern) - Revision history

Jakob Suckale: categories

2009-04-16T14:13:12Z

Jakob Suckale at 14:12, 16 April 2009

2009-04-16T14:12:24Z

←Older revision		Revision as of 14:12, 16 April 2009
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	The '''DNA blot''' or '''Southern blot''' is a molecular biology method to probe the genome for the location of a DNA sequence. Genomic DNA is first digested using a [[restriction endonuclease]]. Fragments are then separated by size using [[agarose gel electrophoresis]]. After size separation DNA pieces are blotted onto a membrane and then hybridised with a labelled nucleic acid probe. The location of the probe on the membrane is visualised either by radioactivity or antibody labelling.		The '''DNA blot''' or '''Southern blot''' is a molecular biology method to probe the genome for the location of a DNA sequence. Genomic DNA is first digested using a [[restriction endonuclease]]. Fragments are then separated by size using [[agarose gel electrophoresis]]. After size separation DNA pieces are blotted onto a membrane and then hybridised with a labelled nucleic acid probe. The location of the probe on the membrane is visualised either by radioactivity or antibody labelling.

-	== Lab-specific ~~DNA blotting~~ protocols ==	+	== Lab-specific protocols ==
	* [[Oneill_Lab:Southern_Blot]]		* [[Oneill_Lab:Southern_Blot]]

Jakob Suckale: lab-specific protocols

2009-04-16T14:12:08Z

lab-specific protocols

←Older revision		Revision as of 14:12, 16 April 2009
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	The '''DNA blot''' or '''Southern blot''' is a molecular biology method to probe the genome for the location of a DNA sequence. Genomic DNA is first digested using a [[restriction endonuclease]]. Fragments are then separated by size using [[agarose gel electrophoresis]]. After size separation DNA pieces are blotted onto a membrane and then hybridised with a labelled nucleic acid probe. The location of the probe on the membrane is visualised either by radioactivity or antibody labelling.		The '''DNA blot''' or '''Southern blot''' is a molecular biology method to probe the genome for the location of a DNA sequence. Genomic DNA is first digested using a [[restriction endonuclease]]. Fragments are then separated by size using [[agarose gel electrophoresis]]. After size separation DNA pieces are blotted onto a membrane and then hybridised with a labelled nucleic acid probe. The location of the probe on the membrane is visualised either by radioactivity or antibody labelling.
		+
		+	== Lab-specific DNA blotting protocols ==
		+	* [[Oneill_Lab:Southern_Blot]]

	== See also ==		== See also ==
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	* [http://www.youtube.com/watch?v=zYSPnSwT6LQ Principle of DNA blot explained in a video]		* [http://www.youtube.com/watch?v=zYSPnSwT6LQ Principle of DNA blot explained in a video]
	* [http://www.bio.davidson.edu/COURSES/GENOMICS/method/Southernblot.html DNA blot overview at Davidson]		* [http://www.bio.davidson.edu/COURSES/GENOMICS/method/Southernblot.html DNA blot overview at Davidson]
		+	* [http://www.dmd.nl/protocol/DNAhybridization_ALG005.html detailed DNA blot protocol from Leiden University]
	* [http://en.wikipedia.org/wiki/Southern_blot DNA blot at Wikipedia]		* [http://en.wikipedia.org/wiki/Southern_blot DNA blot at Wikipedia]
	* [http://en.wikipedia.org/wiki/Electroblotting Electroblotting at Wikipedia]		* [http://en.wikipedia.org/wiki/Electroblotting Electroblotting at Wikipedia]

Jakob Suckale: external links

2009-04-16T14:08:56Z

external links

←Older revision		Revision as of 14:08, 16 April 2009
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	The '''DNA blot''' or '''Southern blot''' is a molecular biology method to probe the genome for the location of a DNA sequence. Genomic DNA is first digested using a [[restriction endonuclease]]. Fragments are then separated by size using [[agarose gel electrophoresis]]. After size separation DNA pieces are blotted onto a membrane and then hybridised with a labelled nucleic acid probe. The location of the probe on the membrane is visualised either by radioactivity or antibody labelling.		The '''DNA blot''' or '''Southern blot''' is a molecular biology method to probe the genome for the location of a DNA sequence. Genomic DNA is first digested using a [[restriction endonuclease]]. Fragments are then separated by size using [[agarose gel electrophoresis]]. After size separation DNA pieces are blotted onto a membrane and then hybridised with a labelled nucleic acid probe. The location of the probe on the membrane is visualised either by radioactivity or antibody labelling.
-

	== See also ==		== See also ==
	* [[RNA blot (Northern)]]		* [[RNA blot (Northern)]]
	* [[Protein blot (Western)]]		* [[Protein blot (Western)]]
		+
		+	== External links ==
		+	* [http://www.youtube.com/watch?v=zYSPnSwT6LQ Principle of DNA blot explained in a video]
		+	* [http://www.bio.davidson.edu/COURSES/GENOMICS/method/Southernblot.html DNA blot overview at Davidson]
		+	* [http://en.wikipedia.org/wiki/Southern_blot DNA blot at Wikipedia]
		+	* [http://en.wikipedia.org/wiki/Electroblotting Electroblotting at Wikipedia]

Jakob Suckale: see also

2009-04-16T14:00:26Z

Jakob Suckale: lead section

2009-04-16T13:48:07Z

lead section

←Older revision		Revision as of 13:48, 16 April 2009
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-	The '''DNA blot''' or '''Southern blot''' is a molecular biology method to probe the genome for the location of a DNA sequence.	+	The '''DNA blot''' or '''Southern blot''' is a molecular biology method to probe the genome for the location of a DNA sequence. Genomic DNA is first digested using a [[restriction endonuclease]]. Fragments are then separated by size using [[agarose gel electrophoresis]]. After size separation DNA pieces are blotted onto a membrane and then hybridised with a labelled nucleic acid probe. The location of the probe on the membrane is visualised either by radioactivity or antibody labelling.

Jakob Suckale: * page created *

2009-04-16T13:41:56Z

*** page created ***

New page

The '''DNA blot''' or '''Southern blot''' is a molecular biology method to probe the genome for the location of a DNA sequence.

←Older revision		Revision as of 14:13, 16 April 2009
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	* [http://en.wikipedia.org/wiki/Southern_blot DNA blot at Wikipedia]		* [http://en.wikipedia.org/wiki/Southern_blot DNA blot at Wikipedia]
	* [http://en.wikipedia.org/wiki/Electroblotting Electroblotting at Wikipedia]		* [http://en.wikipedia.org/wiki/Electroblotting Electroblotting at Wikipedia]
		+
		+	[[Category:Protocol]]
		+	[[Category:DNA]]
		+	[[Category:in vitro]]

←Older revision		Revision as of 14:00, 16 April 2009
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	The '''DNA blot''' or '''Southern blot''' is a molecular biology method to probe the genome for the location of a DNA sequence. Genomic DNA is first digested using a [[restriction endonuclease]]. Fragments are then separated by size using [[agarose gel electrophoresis]]. After size separation DNA pieces are blotted onto a membrane and then hybridised with a labelled nucleic acid probe. The location of the probe on the membrane is visualised either by radioactivity or antibody labelling.		The '''DNA blot''' or '''Southern blot''' is a molecular biology method to probe the genome for the location of a DNA sequence. Genomic DNA is first digested using a [[restriction endonuclease]]. Fragments are then separated by size using [[agarose gel electrophoresis]]. After size separation DNA pieces are blotted onto a membrane and then hybridised with a labelled nucleic acid probe. The location of the probe on the membrane is visualised either by radioactivity or antibody labelling.
		+
		+
		+	== See also ==
		+	* [[RNA blot (Northern)]]
		+	* [[Protein blot (Western)]]