DNA blot (Southern)

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The '''DNA blot''' or '''Southern blot''' is a molecular biology method to probe the genome for the location of a DNA sequence. Genomic DNA is first digested using a [[restriction endonuclease]]. Fragments are then separated by size using [[agarose gel electrophoresis]]. After size separation DNA pieces are blotted onto a membrane and then hybridised with a labelled nucleic acid probe. The location of the probe on the membrane is visualised either by radioactivity or antibody labelling.
The '''DNA blot''' or '''Southern blot''' is a molecular biology method to probe the genome for the location of a DNA sequence. Genomic DNA is first digested using a [[restriction endonuclease]]. Fragments are then separated by size using [[agarose gel electrophoresis]]. After size separation DNA pieces are blotted onto a membrane and then hybridised with a labelled nucleic acid probe. The location of the probe on the membrane is visualised either by radioactivity or antibody labelling.
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== See also ==
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* [[RNA blot (Northern)]]
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* [[Protein blot (Western)]]

Revision as of 09:00, 16 April 2009

The DNA blot or Southern blot is a molecular biology method to probe the genome for the location of a DNA sequence. Genomic DNA is first digested using a restriction endonuclease. Fragments are then separated by size using agarose gel electrophoresis. After size separation DNA pieces are blotted onto a membrane and then hybridised with a labelled nucleic acid probe. The location of the probe on the membrane is visualised either by radioactivity or antibody labelling.


See also

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