DNA blot (Southern): Difference between revisions
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* [http://en.wikipedia.org/wiki/Southern_blot DNA blot at Wikipedia] | * [http://en.wikipedia.org/wiki/Southern_blot DNA blot at Wikipedia] | ||
* [http://en.wikipedia.org/wiki/Electroblotting Electroblotting at Wikipedia] | * [http://en.wikipedia.org/wiki/Electroblotting Electroblotting at Wikipedia] | ||
[[Category:Protocol]] | |||
[[Category:DNA]] | |||
[[Category:in vitro]] | |||
Latest revision as of 07:13, 16 April 2009
The DNA blot or Southern blot is a molecular biology method to probe the genome for the location of a DNA sequence. Genomic DNA is first digested using a restriction endonuclease. Fragments are then separated by size using agarose gel electrophoresis. After size separation DNA pieces are blotted onto a membrane and then hybridised with a labelled nucleic acid probe. The location of the probe on the membrane is visualised either by radioactivity or antibody labelling.
