DNA Precipitation
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Overview
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Materials
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- 3M NaOAc pH 5.2
- EtOH 95%
- Glycogen (optional)
Procedure
- 1/10 volume NaOAc
- 2 volumes EtOH
- -20°C overnight or 30min -70°C.
- Centrifuge >12k G for at least 15 mins.
Notes
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References
Relevant papers and books
- Goldbeter A and Koshland DE Jr. An amplified sensitivity arising from covalent modification in biological systems. Proc Natl Acad Sci U S A. 1981 Nov;78(11):6840-4. DOI:10.1073/pnas.78.11.6840 |
- JACOB F and MONOD J. Genetic regulatory mechanisms in the synthesis of proteins. J Mol Biol. 1961 Jun;3:318-56. DOI:10.1016/s0022-2836(61)80072-7 |
- ISBN:0879697164
Contact
- Who has experience with this protocol?
or instead, discuss this protocol.