DNA Precipitation: Difference between revisions
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(New page: ==Overview== Replace this sentence with a brief description of the protocol and its goal. ==Materials== List reagents, supplies and equipment necessary to perform the protocol here. For...) |
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List reagents, supplies and equipment necessary to perform the protocol here. For those materials which have their own OWW pages, link to that page. Alternatively, links to the suppliers' page on that material are also appropriate. | List reagents, supplies and equipment necessary to perform the protocol here. For those materials which have their own OWW pages, link to that page. Alternatively, links to the suppliers' page on that material are also appropriate. | ||
* | *3M NaOAc pH 5.2 | ||
* | *EtOH 95% | ||
* | *Glycogen (optional) | ||
==Procedure== | ==Procedure== | ||
# | #1/10 volume NaOAc | ||
# | #2 volumes EtOH | ||
# | #-20°C overnight or 30min -70°C. | ||
# | #Centrifuge >12k G for at least 15 mins. | ||
==Notes== | ==Notes== | ||
Revision as of 14:10, 26 July 2007
Overview
Replace this sentence with a brief description of the protocol and its goal.
Materials
List reagents, supplies and equipment necessary to perform the protocol here. For those materials which have their own OWW pages, link to that page. Alternatively, links to the suppliers' page on that material are also appropriate.
- 3M NaOAc pH 5.2
- EtOH 95%
- Glycogen (optional)
Procedure
- 1/10 volume NaOAc
- 2 volumes EtOH
- -20°C overnight or 30min -70°C.
- Centrifuge >12k G for at least 15 mins.
Notes
- List troubleshooting tips here.
- You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
- Anecdotal observations that might be of use to others can also be posted here.
Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.
References
Relevant papers and books
- Goldbeter A and Koshland DE Jr. An amplified sensitivity arising from covalent modification in biological systems. Proc Natl Acad Sci U S A. 1981 Nov;78(11):6840-4. DOI:10.1073/pnas.78.11.6840 |
- JACOB F and MONOD J. Genetic regulatory mechanisms in the synthesis of proteins. J Mol Biol. 1961 Jun;3:318-56. DOI:10.1016/s0022-2836(61)80072-7 |
- ISBN:0879697164
Contact
- Who has experience with this protocol?
or instead, discuss this protocol.
