DMPC - Revision history

Peter R. Oxley at 21:29, 3 August 2011

Peter R. Oxley — Wed, 03 Aug 2011 21:29:02 GMT

←Older revision		Revision as of 21:29, 3 August 2011
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-	DMPC is an acronym for di-methyl~~-propyl carbonate~~.	+	[[Image:MolecularFormulaDMPC.gif\|thumb\|dimethyl pyrocarbonate]]
		+
		+	DMPC is an acronym for di-methyl pyrocarbonate (AKA: dimethyl dicarbonate, pyrocarbonic acid dimethyl ester).

	==Purpose==		==Purpose==
Line 16:		Line 18:
	==Safety==		==Safety==

-	*DMPC is a safer alternative to DEPC (known carcinogen).	+	*DMPC is a safer alternative to [[DEPC]] (known carcinogen).

	==References==		==References==

Reshma P. Shetty at 14:50, 18 November 2009

Reshma P. Shetty — Wed, 18 Nov 2009 14:50:57 GMT

←Older revision		Revision as of 14:50, 18 November 2009
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	*Treatment involves adding DMPC to 0.1% v/v and incubating at 37°C for 1 hour to overnight followed by autoclaving. Autoclaving destroys DMPC and is an essential step. Esters may be generated during autoclaving giving rise to a 'fruity' smell (that is not coming directly from DMPC).		*Treatment involves adding DMPC to 0.1% v/v and incubating at 37°C for 1 hour to overnight followed by autoclaving. Autoclaving destroys DMPC and is an essential step. Esters may be generated during autoclaving giving rise to a 'fruity' smell (that is not coming directly from DMPC).

-	*Note that DMPC cannot be used with chemical solutions that have amine groups, such as Tris and HEPES buffers, or mercaptans. In such cases, use ~~DEPC~~-treated water to generate the solution.	+	*Note that DMPC cannot be used with chemical solutions that have amine groups, such as Tris and HEPES buffers, or mercaptans. In such cases, use DMPC-treated water to generate the solution.

	==Safety==		==Safety==

Reshma P. Shetty at 14:48, 18 November 2009

Reshma P. Shetty — Wed, 18 Nov 2009 14:48:45 GMT

←Older revision		Revision as of 14:48, 18 November 2009
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	*Treatment involves adding DMPC to 0.1% v/v and incubating at 37°C for 1 hour to overnight followed by autoclaving. Autoclaving destroys DMPC and is an essential step. Esters may be generated during autoclaving giving rise to a 'fruity' smell (that is not coming directly from DMPC).		*Treatment involves adding DMPC to 0.1% v/v and incubating at 37°C for 1 hour to overnight followed by autoclaving. Autoclaving destroys DMPC and is an essential step. Esters may be generated during autoclaving giving rise to a 'fruity' smell (that is not coming directly from DMPC).

-	*Note that DMPC cannot be used with chemical solutions that have amine groups, such as Tris and HEPES buffers, or mercaptans. In such cases, use ~~DMPC~~-treated water to generate the solution.	+	*Note that DMPC cannot be used with chemical solutions that have amine groups, such as Tris and HEPES buffers, or mercaptans. In such cases, use DEPC-treated water to generate the solution.

	==Safety==		==Safety==

Torsten Waldminghaus: adapted to template and added some details

Torsten Waldminghaus — Thu, 06 Mar 2008 14:17:53 GMT

adapted to template and added some details

←Older revision		Revision as of 14:17, 6 March 2008
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-	DMPC is an acronym for di-methyl-propyl carbonate. ~~It is typically used to treat water to remove RNases.~~	+	DMPC is an acronym for di-methyl-propyl carbonate.

-	DMPC reacts with amine, hydroxy and thiol groups of proteins (such as RNAses) and inactivates RNAses. Treatment involves adding DMPC to 0.1% v/v and incubating at 37°C for 1 hour to overnight followed by autoclaving. Autoclaving destroys DMPC and is an essential step. Esters may be generated during autoclaving giving rise to a 'fruity' smell (that is not coming directly from DMPC).	+	==Purpose==
		+	*DMPC is typically used to treat water to remove RNases. It reacts with amine, hydroxy and thiol groups of proteins (such as RNAses) and inactivates RNAses.

-	~~Note that~~ DMPC ~~cannot be used with chemical solutions that have amine groups, such as Tris and HEPES buffers, or mercaptans~~. ~~In such cases, use DMPC-treated water to generate the solution~~.	+	==Procurement==
		+	*You can buy DMPC for example at [http://www.sigmaaldrich.com Sigma].

-	DMPC is a safer alternative to [[DEPC]] (known carcinogen). It is used in exactly the same way.	+	==Use==
		+	*DMPC is a safer alternative to [[DEPC]] (known carcinogen). It is used in exactly the same way.
		+
		+	*Treatment involves adding DMPC to 0.1% v/v and incubating at 37°C for 1 hour to overnight followed by autoclaving. Autoclaving destroys DMPC and is an essential step. Esters may be generated during autoclaving giving rise to a 'fruity' smell (that is not coming directly from DMPC).
		+
		+	*Note that DMPC cannot be used with chemical solutions that have amine groups, such as Tris and HEPES buffers, or mercaptans. In such cases, use DMPC-treated water to generate the solution.
		+
		+	==Safety==
		+
		+	*DMPC is a safer alternative to DEPC (known carcinogen).

	==References==		==References==
	#[http://stanxterm.aecom.yu.edu/wiki/index.php?page=RNA_handling Handling RNA]		#[http://stanxterm.aecom.yu.edu/wiki/index.php?page=RNA_handling Handling RNA]
	#[http://www.ambion.com/techlib/tb/tb_178.html RNase and DEPC Treatment: Fact or Laboratory Myth]		#[http://www.ambion.com/techlib/tb/tb_178.html RNase and DEPC Treatment: Fact or Laboratory Myth]
		+

	[[Category:Material]]		[[Category:Material]]
	[[Category:RNA]]		[[Category:RNA]]
	[[Category:Chemical]]		[[Category:Chemical]]

Reshma P. Shetty at 17:30, 19 October 2007

Reshma P. Shetty — Fri, 19 Oct 2007 17:30:13 GMT

←Older revision		Revision as of 17:30, 19 October 2007
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	[[Category:Material]]		[[Category:Material]]
	[[Category:RNA]]		[[Category:RNA]]
		+	[[Category:Chemical]]

Reshma P. Shetty at 19:29, 10 December 2006

Reshma P. Shetty — Sun, 10 Dec 2006 19:29:56 GMT

←Older revision		Revision as of 19:29, 10 December 2006
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	#[http://stanxterm.aecom.yu.edu/wiki/index.php?page=RNA_handling Handling RNA]		#[http://stanxterm.aecom.yu.edu/wiki/index.php?page=RNA_handling Handling RNA]
	#[http://www.ambion.com/techlib/tb/tb_178.html RNase and DEPC Treatment: Fact or Laboratory Myth]		#[http://www.ambion.com/techlib/tb/tb_178.html RNase and DEPC Treatment: Fact or Laboratory Myth]
		+
		+	[[Category:Material]]
		+	[[Category:RNA]]

Reshma P. Shetty at 18:49, 8 December 2006

Reshma P. Shetty — Fri, 08 Dec 2006 18:49:14 GMT

New page

DMPC is an acronym for di-methyl-propyl carbonate. It is typically used to treat water to remove RNases.

DMPC reacts with amine, hydroxy and thiol groups of proteins (such as RNAses) and inactivates RNAses. Treatment involves adding DMPC to 0.1% v/v and incubating at 37°C for 1 hour to overnight followed by autoclaving. Autoclaving destroys DMPC and is an essential step. Esters may be generated during autoclaving giving rise to a 'fruity' smell (that is not coming directly from DMPC).

Note that DMPC cannot be used with chemical solutions that have amine groups, such as Tris and HEPES buffers, or mercaptans. In such cases, use DMPC-treated water to generate the solution.

DMPC is a safer alternative to [[DEPC]] (known carcinogen). It is used in exactly the same way.

==References==
#[http://stanxterm.aecom.yu.edu/wiki/index.php?page=RNA_handling Handling RNA]
#[http://www.ambion.com/techlib/tb/tb_178.html RNase and DEPC Treatment: Fact or Laboratory Myth]