DMPC: Difference between revisions
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[[Image:MolecularFormulaDMPC.gif|thumb|dimethyl pyrocarbonate]] | |||
DMPC | DMPC is an acronym for di-methyl pyrocarbonate (AKA: dimethyl dicarbonate, pyrocarbonic acid dimethyl ester). | ||
==Purpose== | |||
*DMPC is typically used to treat water to remove RNases. It reacts with amine, hydroxy and thiol groups of proteins (such as RNAses) and inactivates RNAses. | |||
DMPC is a safer alternative to [[DEPC]] (known carcinogen). It is used in exactly the same way. | ==Procurement== | ||
*You can buy DMPC for example at [http://www.sigmaaldrich.com Sigma]. | |||
==Use== | |||
*DMPC is a safer alternative to [[DEPC]] (known carcinogen). It is used in exactly the same way. | |||
*Treatment involves adding DMPC to 0.1% v/v and incubating at 37°C for 1 hour to overnight followed by autoclaving. Autoclaving destroys DMPC and is an essential step. Esters may be generated during autoclaving giving rise to a 'fruity' smell (that is not coming directly from DMPC). | |||
*Note that DMPC cannot be used with chemical solutions that have amine groups, such as Tris and HEPES buffers, or mercaptans. In such cases, use DMPC-treated water to generate the solution. | |||
==Safety== | |||
*DMPC is a safer alternative to [[DEPC]] (known carcinogen). | |||
==References== | ==References== | ||
#[http://stanxterm.aecom.yu.edu/wiki/index.php?page=RNA_handling Handling RNA] | #[http://stanxterm.aecom.yu.edu/wiki/index.php?page=RNA_handling Handling RNA] | ||
#[http://www.ambion.com/techlib/tb/tb_178.html RNase and DEPC Treatment: Fact or Laboratory Myth] | #[http://www.ambion.com/techlib/tb/tb_178.html RNase and DEPC Treatment: Fact or Laboratory Myth] | ||
[[Category:Material]] | [[Category:Material]] | ||
[[Category:RNA]] | [[Category:RNA]] | ||
[[Category:Chemical]] | |||
Latest revision as of 14:29, 3 August 2011
DMPC is an acronym for di-methyl pyrocarbonate (AKA: dimethyl dicarbonate, pyrocarbonic acid dimethyl ester).
Purpose
- DMPC is typically used to treat water to remove RNases. It reacts with amine, hydroxy and thiol groups of proteins (such as RNAses) and inactivates RNAses.
Procurement
- You can buy DMPC for example at Sigma.
Use
- DMPC is a safer alternative to DEPC (known carcinogen). It is used in exactly the same way.
- Treatment involves adding DMPC to 0.1% v/v and incubating at 37°C for 1 hour to overnight followed by autoclaving. Autoclaving destroys DMPC and is an essential step. Esters may be generated during autoclaving giving rise to a 'fruity' smell (that is not coming directly from DMPC).
- Note that DMPC cannot be used with chemical solutions that have amine groups, such as Tris and HEPES buffers, or mercaptans. In such cases, use DMPC-treated water to generate the solution.
Safety
- DMPC is a safer alternative to DEPC (known carcinogen).
