DMPC

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DMPC is an acronym for di-methyl-propyl carbonate.  It is typically used to treat water to remove RNases.
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[[Image:MolecularFormulaDMPC.gif|thumb|dimethyl pyrocarbonate]]
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DMPC reacts with amine, hydroxy and thiol groups of proteins (such as RNAses) and inactivates RNAses. Treatment involves adding DMPC to 0.1% v/v and incubating at 37°C for 1 hour to overnight followed by autoclaving. Autoclaving destroys DMPC and is an essential step.  Esters may be generated during autoclaving giving rise to a 'fruity' smell (that is not coming directly from DMPC).
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DMPC is an acronym for di-methyl pyrocarbonate (AKA: dimethyl dicarbonate, pyrocarbonic acid dimethyl ester).  
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Note that DMPC cannot be used with chemical solutions that have amine groups, such as Tris and HEPES buffers, or mercaptans.  In such cases, use DMPC-treated water to generate the solution.
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==Purpose==
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*DMPC is typically used to treat water to remove RNases. It reacts with amine, hydroxy and thiol groups of proteins (such as RNAses) and inactivates RNAses.
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DMPC is a safer alternative to [[DEPC]] (known carcinogen). It is used in exactly the same way.
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==Procurement==
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*You can buy DMPC for example at [http://www.sigmaaldrich.com Sigma].
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==Use==
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*DMPC is a safer alternative to [[DEPC]] (known carcinogen). It is used in exactly the same way.
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*Treatment involves adding DMPC to 0.1% v/v and incubating at 37°C for 1 hour to overnight followed by autoclaving. Autoclaving destroys DMPC and is an essential step. Esters may be generated during autoclaving giving rise to a 'fruity' smell (that is not coming directly from DMPC).
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*Note that DMPC cannot be used with chemical solutions that have amine groups, such as Tris and HEPES buffers, or mercaptans. In such cases, use DMPC-treated water to generate the solution.
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==Safety==
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*DMPC is a safer alternative to [[DEPC]] (known carcinogen).
==References==
==References==
#[http://stanxterm.aecom.yu.edu/wiki/index.php?page=RNA_handling Handling RNA]
#[http://stanxterm.aecom.yu.edu/wiki/index.php?page=RNA_handling Handling RNA]
#[http://www.ambion.com/techlib/tb/tb_178.html RNase and DEPC Treatment: Fact or Laboratory Myth]
#[http://www.ambion.com/techlib/tb/tb_178.html RNase and DEPC Treatment: Fact or Laboratory Myth]
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[[Category:Material]]
[[Category:Material]]
[[Category:RNA]]
[[Category:RNA]]
[[Category:Chemical]]
[[Category:Chemical]]

Current revision

dimethyl pyrocarbonate
dimethyl pyrocarbonate

DMPC is an acronym for di-methyl pyrocarbonate (AKA: dimethyl dicarbonate, pyrocarbonic acid dimethyl ester).

Contents

Purpose

  • DMPC is typically used to treat water to remove RNases. It reacts with amine, hydroxy and thiol groups of proteins (such as RNAses) and inactivates RNAses.

Procurement

  • You can buy DMPC for example at Sigma.

Use

  • DMPC is a safer alternative to DEPC (known carcinogen). It is used in exactly the same way.
  • Treatment involves adding DMPC to 0.1% v/v and incubating at 37°C for 1 hour to overnight followed by autoclaving. Autoclaving destroys DMPC and is an essential step. Esters may be generated during autoclaving giving rise to a 'fruity' smell (that is not coming directly from DMPC).
  • Note that DMPC cannot be used with chemical solutions that have amine groups, such as Tris and HEPES buffers, or mercaptans. In such cases, use DMPC-treated water to generate the solution.

Safety

  • DMPC is a safer alternative to DEPC (known carcinogen).

References

  1. Handling RNA
  2. RNase and DEPC Treatment: Fact or Laboratory Myth
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