DIYbio/BOSSlab/Notebook/2011/05/15: Difference between revisions
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* incubating one 1.5 mL tube with remaining transformant (900 uL) at 37 C. no shaking. | * incubating one 1.5 mL tube with remaining transformant (900 uL) at 37 C. no shaking. | ||
* storing the other 1.5 mL tube with remaining transformant (900 uL) at 4 C. | * storing the other 1.5 mL tube with remaining transformant (900 uL) at 4 C. | ||
* incubation started at | * incubation started at 10:57 PM 5/15/2011 | ||
* the incubator is very erratic. I'm trying to keep the temperature at or below 37 C. It does not have a very sensitive control loop. I think it is designed to stay +/- 10 C its set point. It's really more of an oven. | |||
Revision as of 19:58, 15 May 2011
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html> |
Photos: http://www.flickr.com/photos/macowell/sets/72157626730414854/ Preflightwhat's our goal: transformation of Invivogen lyocomp cells w/ BBa_J04450 on card (circa 2008). Materials
what are the risks to us
what waste are we gonna generate
mitigation of community risks
Protocolbased on http://www.invivogen.com/PDF/LyoComp_GT116_TDS.pdf
Notes
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