DEPC

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==External links==
==External links==
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#[http://stanxterm.aecom.yu.edu/wiki/index.php?page=RNA_handling Handling RNA]
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*[http://stanxterm.aecom.yu.edu/wiki/index.php?page=RNA_handling Handling RNA from Stanley lab, Yeshiva Uni, NY]
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#[http://www.ambion.com/techlib/tb/tb_178.html RNase and DEPC Treatment: Fact or Laboratory Myth]
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*[http://www.ambion.com/techlib/tb/tb_178.html RNase and DEPC Treatment: Fact or Laboratory Myth]
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#[https://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/D5758 Molecular biology grade DEPC from Sigma]
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*[https://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/D5758 Molecular biology grade DEPC from Sigma]
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*[http://www.ncbi.nlm.nih.gov/pubmed/8643347 Comparison of DEPC-treated water and Milli-Q PF Plus water] (similar RNA degradation in Milli-Q and DEPC-treated water)
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DEPC-related discussion threads:
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* [http://www.google.de/search?hl=en&lr=&ie=ISO-8859-1&q=site%3Awww.protocol-online.org%20DEPC&btnG=Google+Search Bioforum (protocol-online.org) searched for DEPC]
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* [http://www.google.com/search?q=site%3Awww.bio.net%2Fbionet%2Fmm%2Fmethods&q=DEPC Methods forum on Bio.net searched for DEPC]
[[Category:Material]]
[[Category:Material]]
[[Category:RNA]]
[[Category:RNA]]
[[Category:Chemical]]
[[Category:Chemical]]

Revision as of 12:33, 29 January 2009

chemical structure of diethyl pyrocarbonate (DEPC)
chemical structure of diethyl pyrocarbonate (DEPC)

Diethyl pyrocarbonate (DEPC) is an efficient, nonspecific inhibitor of RNases. It is typically used to treat water and solutions before working with easily degraded RNA. DEPC reacts with amine, hydroxy and thiol groups of proteins thereby inactivating RNAses (and other enzymes).

Contents

Procurement

  • 100ml DEPC from Sigma (D5758) [1] - €233/$292 (as of 2009-01)
  • 100ml DEPC from MPI (150902) [2] - €313 (as of 2009-01)

Use

  • Treatment involves adding DEPC to 0.1% v/v and incubating at 37°C for 1 hour to overnight followed by autoclaving. Autoclaving destroys DEPC and is an essential step. Esters may be generated during autoclaving giving rise to a 'fruity' smell (that is not coming directly from DEPC).
  • Note that DEPC cannot be used with chemical solutions that have amine groups, such as Tris and HEPES buffers, or mercaptans. In such cases, use DEPC-treated water to generate the solution.

Safety

  • DEPC is carcinogenic (it reacts with adenine) and should be handled with care. Wear gloves!
  • Di-methyl-propyl carbonate (DMPC), a safer alternative to DEPC, is used in exactly the same way.

Internal links

External links

DEPC-related discussion threads:

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