Etchevers at 11:45, 9 February 2006

Etchevers — Thu, 09 Feb 2006 11:45:28 GMT

←Older revision		Revision as of 11:45, 9 February 2006
Line 3:		Line 3:
	*stains both live and dead cells		*stains both live and dead cells
	*can be excited with a mercury-arc lamp or with the UV lines of the argon-ion laser		*can be excited with a mercury-arc lamp or with the UV lines of the argon-ion laser
-	*better photostability than ~~Hoesht~~ dyes	+	*better photostability than Hoechst dyes
	*blue-fluorescent		*blue-fluorescent
		+
		+
		+	== Safety information: ==
		+
		+	English: Diamidino-4', 6-phenylindole-2 or DAPI is not classed as a dangerous substance in Europe according to European directive 67/548. However, for biomedical use it is considered to be an irritant (label Xi R36/37/38). Nitrile gloves of 0.11mm thickness ensures protection. Because of its intercalating nature it is disposed of with dangerous substances aside from carcinogens.
		+
		+	(Wear gloves and consider disposal in the same way you dispose of ethidium bromide).
		+
		+	French: Le dichlorhydrate de Diamidino-4',6-phénylindole-2 ou DAPI n'est pas classé comme substance dangereuse en Europe au sens de la Directive 67/548. A l'Inserm, nous le classons irritant (Xi R36/37/38). Le port de gants en caoutchouc nitrile de 0.11 mm assure un temps de traversée > 480 min. En raison de son caractère intercalant on peut le faire éliminer avec les produits dangereux (non cancérogènes)

Reshma P. Shetty: DAPI

Reshma P. Shetty — Thu, 28 Apr 2005 17:11:48 GMT

DAPI

New page

*358/461
*double stranded nucleic acid dye, associates with the minor groove of dsDNA, preferentially binds AT clusters
*stains both live and dead cells
*can be excited with a mercury-arc lamp or with the UV lines of the argon-ion laser
*better photostability than Hoesht dyes
*blue-fluorescent

DAPI - Revision history

Etchevers at 11:45, 9 February 2006

Reshma P. Shetty: DAPI