Critical micelle concentration (CMC): Difference between revisions
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* [http://en.wikipedia.org/wiki/Critical_micelle_concentration Wikipedia: Critical micelle concentration] | * [http://en.wikipedia.org/wiki/Critical_micelle_concentration Wikipedia: Critical micelle concentration] | ||
* [http://www.antibodybeyond.com/books/Calbiochem_Detergents_Booklet.pdf old (2001) Calbiochem Detergent brochure] | * [http://www.antibodybeyond.com/books/Calbiochem_Detergents_Booklet.pdf old (2001) Calbiochem Detergent brochure] | ||
* [http://mrsec.wisc.edu/Edetc/nanolab/micelle/index.html Explanation and Video showing an educational experiment to determine the CMC of SDS, Uni Wisconsin] | |||
[[Category:Material]] | [[Category:Material]] | ||
[[Category:Detergents]] | [[Category:Detergents]] |
Revision as of 13:01, 29 July 2009
Critical micelle concentration (CMC) is defined as the concentration of detergents above which micelles are spontaneously formed. The CMC is important in biology because at concentrations above it the detergents form complexes with lipophilic proteins. Below this borderline, detergents merely partition into membranes without solubilising membrane proteins.
Detergent | CMC (%w/v) | CMC (mM) | MW | Type |
---|---|---|---|---|
BRIJ 35 | 0.11 | 0.09 | 1200 | non-ionic |
NP-40 | 0.017 | 0.3 | ~650 | non-ionic |
Saponin | ~0.1 | mixture | non-ionic | |
Triton X-100 | 0.021 | 0.3 | ~650 | non-ionic |
Tween 20 | 0.073 | 0.059 | ~1228 | non-ionic |
SDS | 0.23 | 7-10 | 288.5 | ionic |
CHAPS | 0.49 | 6-10 | 615 | zwitterionic |
Note: The molecular weights for some detergents are average values. Triton X-100, for example, can range between 600 and over 650 MW depending on synthesis. The exact molecular weight influences the CMC.
Note: Saponin is a class of amphiphilic, natural metabolites with detergent properties often extracted from plants. CMC may vary.
Sources
- new, abbreviated Detergent guide, Calbiochem
- Commonly used detergents, Frost lecture, UFL
- Urum et al 2004, incl. data on saponin