Crisanti:Materials

From OpenWetWare

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Enzyme Kits:

• BD Advantage 2 PCR Kit

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• Phusion™ High-Fidelity DNA Polymerase

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• MEGAscript® High Yield Transcription Kit 25rxn

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• MEGAclear® Kit 20rxn

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Cloning Kits:

• pGEM®-T Easy Vector System I

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• pET101 TOPO Expression Kit

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Other Kits:

• QIAgen Plasmid Maxi Kit (25)

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• QIAquick gel extraction kit

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Antibodies:

• Anti-HA Tag mouse Monoclonal
  

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Size Markers:

• Bioline Hyperladder 1 (200-10 000 bp)
  

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Radioactivity 32p:

• P32 dATP

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• α-P32 UTP

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You will need a new TAD number first which you can get by emailing Nicola Donaldson

xNTPs & other stuff:

• 8452, 10 mM Bio-16-UTP

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• Biorad Poly-Prep Chromatography Columns, empty 50

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• ImmunoPure Immobilized Streptavidin, 5 ml

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