Cresol Red

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(molecular weight, pH indicator)
Current revision (09:31, 3 June 2009) (view source)
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[[Image:Cresol red.png|right|150px]]
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[[Image:Cresol red.png|right|150px|thumb|chemical structure of cresol red]]
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'''Cresol red''' is a pH indicator and molecular weight marker for agarose gels. Unlike bromophenol blue and xylene cyanol, cresol red does not inhibit Taq polymerase in PCRs.
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[[Image:Agarose gel dyes.jpg|right|150px]]
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'''Cresol red''' is a pH indicator and [[Agarose_gel_loading_dye#Colour|molecular weight marker for agarose gels]]. Unlike [[bromophenol blue]] and [[xylene cyanol]], cresol red does not inhibit Taq polymerase in PCRs.
== Agarose molecular weight marker ==
== Agarose molecular weight marker ==
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* molecular weight 404 g/mol
* molecular weight 404 g/mol
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* in 1% agarose at ~125 bp
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* in 1% agarose at ~1000 bp (between xylene cyanol 5000bp and bromophenol blue 500bp)
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* in 2% agarose at ~300 bp (between xylene cyanol and bromophenol blue)
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* in 3% agarose at >100bp (xylene cyanol 300bp)
== pH indicator ==
== pH indicator ==
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== External links ==
== External links ==
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* [[Image:3stars.png]] [http://www.wendychao.com/science/protocols/loading_buffer/ Wendy Chao's loading dye recipe and discussion]
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* [http://molbiol.ru/eng/protocol/01_03.html#a5 Cresol Red solution calculator]
* [http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi?sid=73160&loc=ec_rcs Cresol red at Pubchem]
* [http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi?sid=73160&loc=ec_rcs Cresol red at Pubchem]
* [http://www.sigmaaldrich.com/catalog/ProductDetail.do?N4=114480|Aldrich&N5=Product%20No.|BRAND_KEY&F=SPEC Cresol Red sodium salt - Sigma catalogue]
* [http://www.sigmaaldrich.com/catalog/ProductDetail.do?N4=114480|Aldrich&N5=Product%20No.|BRAND_KEY&F=SPEC Cresol Red sodium salt - Sigma catalogue]
* [http://www.sigmaaldrich.com/life-science/cell-biology/hematology-and-histology/hematology-and-histology-products.html?TablePage=17895485 Stains and Dyes Letter C of the Sigma Aldrich online catalogue showing different Cresol varieties]  
* [http://www.sigmaaldrich.com/life-science/cell-biology/hematology-and-histology/hematology-and-histology-products.html?TablePage=17895485 Stains and Dyes Letter C of the Sigma Aldrich online catalogue showing different Cresol varieties]  
* [http://en.wikipedia.org/wiki/Cresol_Red Cresol Red at Wikipedia]
* [http://en.wikipedia.org/wiki/Cresol_Red Cresol Red at Wikipedia]
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[[Category:Material]] [[Category:Chemical]] [[Category:agarose gel electrophoresis]]

Current revision

chemical structure of cresol red
chemical structure of cresol red

Cresol red is a pH indicator and molecular weight marker for agarose gels. Unlike bromophenol blue and xylene cyanol, cresol red does not inhibit Taq polymerase in PCRs.

Agarose molecular weight marker

advantage: much less shadow on EtBr pictures unlike xylene cyanol and bromophenol blue

  • molecular weight 404 g/mol
  • in 1% agarose at ~1000 bp (between xylene cyanol 5000bp and bromophenol blue 500bp)
  • in 3% agarose at >100bp (xylene cyanol 300bp)

pH indicator

  • amber - acidic pH
  • yellow - neutral
  • red - alkaline

External links

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